Next Article in Journal
Isolation of Monoterpene Dihydrochalcones from Piper montealegreanum Yuncker (Piperaceae)
Next Article in Special Issue
Pattern Evolution during Double Liquid-Vapor Phase Transitions under Weightlessness
Previous Article in Journal
Isoprenoids Production from Lipid-Extracted Microalgal Biomass Residues Using Engineered E. coli
Previous Article in Special Issue
Effective Subcritical Butane Extraction of Bifenthrin Residue in Black Tea
Article Menu
Issue 6 (June) cover image

Export Article

Open AccessArticle
Molecules 2017, 22(6), 962; doi:10.3390/molecules22060962

Subcritical Water Chromatography with Electrochemical Detection

Department of Chemistry, East Carolina University, Science & Technology Building 584, Greenville, NC 27858, USA
*
Author to whom correspondence should be addressed.
Received: 9 May 2017 / Revised: 3 June 2017 / Accepted: 7 June 2017 / Published: 9 June 2017
(This article belongs to the Special Issue Sub- and Supercritical Fluids and Green Chemistry)
View Full-Text   |   Download PDF [2159 KB, uploaded 9 June 2017]   |  

Abstract

Reverse phase liquid chromatography (RPLC) is a commonly used separation and analysis technique. RPLC typically employs mixtures of organic solvents and water or aqueous buffers as the mobile phase. With RPLC being used on a global scale, enormous quantities of organic solvents are consumed every day. In addition to the purchasing cost of the hazardous solvents, the issue of waste disposal is another concern. At ambient temperature, water is too polar to dissolve many organic substances. Therefore, although water is nontoxic it cannot be used to replace the mobile phase in RPLC since organic analytes will not be eluted. Subcritical water chromatography may be an alternative. The characteristics of water, such as polarity, surface tension, and viscosity, can be altered by manipulating water’s temperature, thus making it behave like an organic solvent. The aim of this study was to evaluate the feasibility of separation using water mobile phase and detection by an electrochemical (EC) detector. The classes of analytes studied were neurotransmitters/metabolites, nucleic acids/heterocyclic bases, and capsaicinoids. Both isothermal and temperature-programmed separations were carried out. The separation temperature ranged from 25 to 100 °C. For separations of all three classes of solutes, the retention time was decreased with increasing temperature, thus shortening the analysis time. The peaks also became narrower as temperature increased. The limit of detection of neurotransmitters/metabolites ranges from 0.112 to 0.224 ppm. View Full-Text
Keywords: subcritical water chromatography; hot water chromatography; electrochemical detection; neurotransmitters; nucleic acids; heterocyclic bases; capsaicinoids subcritical water chromatography; hot water chromatography; electrochemical detection; neurotransmitters; nucleic acids; heterocyclic bases; capsaicinoids
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Anderson, H.; Yang, Y. Subcritical Water Chromatography with Electrochemical Detection. Molecules 2017, 22, 962.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top