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Molecules 2017, 22(5), 823; doi:10.3390/molecules22050823

Antibacterial Activity of 7-Epiclusianone and Its Novel Copper Metal Complex on Streptococcus spp. Isolated from Bovine Mastitis and Their Cytotoxicity in MAC-T Cells

1
Department of Veterinary, Universidade Federal de Viçosa, Viçosa 36570-900, Brazil
2
Department of Chemistry, Universidade Federal de Viçosa, Viçosa 36570-900, Brazil
3
Department of General Biology, Universidade Federal de Viçosa, Viçosa 36570-900, Brazil
4
Institute of Chemistry, Universidade Federal de Alfenas, Alfenas 37130-000, Brazil
Deceased.
*
Author to whom correspondence should be addressed.
Academic Editor: Kelly Chibale
Received: 23 March 2017 / Revised: 4 May 2017 / Accepted: 10 May 2017 / Published: 17 May 2017
(This article belongs to the Section Medicinal Chemistry)
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Abstract

Mastitis is an inflammation of mammary gland parenchyma that adversely affects bovine health and dairy production worldwide despite significant efforts to eradicate it. The aim of this work was to characterize the antimicrobial activity of 7-epiclusianone (7-epi), a compound extracted from the Rheedia brasiliensis fruit, its complex with copper against Streptococcus spp. isolated from bovine mastitis, and to assess their cytotoxicity to bovine mammary alveolar cells (MAC-T). The complex 7-epiclusianone-Cu (7-epi-Cu) was an amorphous green solid with optical activity. Its vibrational spectrum in the infrared region showed absorption bands in the high-frequency region, as well as bands that can be attributed to the unconjugated and conjugated stretching of the free ligand. The complex was anhydrous. One of the tested bacterial strains was not sensitive to the compounds, while the other three had MIC values of 7.8 µg mL−1 and minimum bactericidal concentration (MBC) values between 15.6 and 31.3 µg mL−1. These two compounds are bacteriostatic, did not cause damage to the cell wall and, at sub-inhibitory concentrations, did not induce bacterial adhesion. The compounds were not cytotoxic. Based on these results, 7-epi and 7-epi-Cu exhibited desirable antimicrobial properties and could potentially be used in bovine mastitis treatment. View Full-Text
Keywords: 7-epiclusianone; mastitis; bacterial activity; cytotoxicity; MAC-T; Streptococcus spp.; Rheedia brasiliensis 7-epiclusianone; mastitis; bacterial activity; cytotoxicity; MAC-T; Streptococcus spp.; Rheedia brasiliensis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

de Barros, M.; Perciano, P.G.; dos Santos, M.H.; De Oliveira, L.L.; Costa, É.D.; Moreira, M.A.S. Antibacterial Activity of 7-Epiclusianone and Its Novel Copper Metal Complex on Streptococcus spp. Isolated from Bovine Mastitis and Their Cytotoxicity in MAC-T Cells. Molecules 2017, 22, 823.

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