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Molecules 2017, 22(5), 814; doi:10.3390/molecules22050814

Optimization of EPS Production and Characterization by a Halophilic Bacterium, Kocuria rosea ZJUQH from Chaka Salt Lake with Response Surface Methodology

1
Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China
2
College of Eco-Environmental Engineering, Qinghai Province, Xining 810016, China
*
Author to whom correspondence should be addressed.
Academic Editor: Quan-Bin Han
Received: 18 April 2017 / Revised: 11 May 2017 / Accepted: 11 May 2017 / Published: 16 May 2017
(This article belongs to the Special Issue Natural Polysaccharides)
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Abstract

With the rising awareness of microbial exopolysaccharides (EPSs) application in various fields, halophilic microorganisms which produce EPSs have received broad attention. A newly identified Kocuria rosea ZJUQH CCTCC M2016754 was determined to be a moderate halobacterium on account of its successful adaption to the environment containing 10% NaCl. The optimal combination of fermentation medium compositions on EPS production was studied. In this work, a fractional factorial design was adopted to investigate the significant factors that affected EPS production. The factors of KCl and MgSO4 were found to have a profound impact on EPS production. We utilized central composite design and response surface methodology to derive a statistical model for optimizing the submerged culture medium composition. Judging from these experimental results, the optimum culture medium for producing EPSs was composed of 0.50% casein hydrolysate, 1.00% sodium citrate, 0.30% yeast extract, 0.50% KCl, 0.50% peptone, and 5.80% MgSO4 (initial pH 7.0). The maximal EPS was 48.01 g/L, which is close to the predicted value (50.39 g/L). In the validation experiment, the highest concentration of 70.64 g/L EPSs was obtained after 120 h under the optimized culture medium in a 5-L bioreactor. EPS from this bacterium was also characterized by differential scanning calorimetry (DSC) and Fourier transform infrared analysis (FT-IR). The findings in this study imply that Kocuria rosea ZJUQH has great potential to be exploited as a source of EPSs utilized in food, the pharmaceutical and agriculture industry, and in the biotreatment of hypersaline environments. View Full-Text
Keywords: halophilic bacterium; Kocuria rosea ZJUQH; exopolysaccharides; optimization; response surface methodology halophilic bacterium; Kocuria rosea ZJUQH; exopolysaccharides; optimization; response surface methodology
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Gu, D.; Jiao, Y.; Wu, J.; Liu, Z.; Chen, Q. Optimization of EPS Production and Characterization by a Halophilic Bacterium, Kocuria rosea ZJUQH from Chaka Salt Lake with Response Surface Methodology. Molecules 2017, 22, 814.

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