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Molecules 2017, 22(5), 747; doi:10.3390/molecules22050747

In Vitro Anticancer Activity and Structural Characterization of Ubiquinones from Antrodia cinnamomea Mycelium

1
Graduate Institute of Medical Science, National Defense Medical Center, No.116, Sec.6, Minchuan East Road, Neihu District, Taipei 114, Taiwan
2
School of Pharmacy, National Defense Medical Center, Taipei 114, Taiwan
3
Graduate Institute of Aerospace and Undersea Medicine, National Defense Medical Center, Taipei 114, Taiwan
4
Department of Radiation Oncology, Tri-Service General Hospital, National Defense Medical Center, Taipei 114, Taiwan
5
LanTyng Biotech, Co, Ltd., Taipei 114, Taiwan
*
Author to whom correspondence should be addressed.
Academic Editor: Stefan Berger
Received: 23 March 2017 / Revised: 22 April 2017 / Accepted: 3 May 2017 / Published: 6 May 2017
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Abstract

Two new ubiquinones, named antrocinnamone and 4-acetylantrocamol LT3, were isolated along with six known ubiquinones from Antrodia cinnamomea (Polyporaceae) mycelium. The developed HPLC analysis methods successfully identified eight different ubiquinones, two benzenoids, and one maleic acid derivative from A. cinnamomea. The ubiquinones 18 exhibited potential and selective cytotoxic activity against three human cancer cell lines, with IC50 values ranging from 0.001 to 35.883 μM. We suggest that the different cytotoxicity levels were related to their chemical structures, especially the 4-hydroxycyclohex-2-enone ring and the presence of a free hydroxyl group in the side chain. The suppression by 4-acetylantrocamol LT3 stopped the cell cycle at the beginning of the G2-M phase thus making the cell cycle arrest at the sub-G1 phase as compared with control cells. View Full-Text
Keywords: Antrodia cinnamomea; ubiquinone derivative; antrocinnamone; 4-acetylantrocamol LT3; HPLC analysis and fingerprint; cytotoxicity; flow cytometry; cell cycle Antrodia cinnamomea; ubiquinone derivative; antrocinnamone; 4-acetylantrocamol LT3; HPLC analysis and fingerprint; cytotoxicity; flow cytometry; cell cycle
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MDPI and ACS Style

Yen, I.-C.; Lee, S.-Y.; Lin, K.-T.; Lai, F.-Y.; Kuo, M.-T.; Chang, W.-L. In Vitro Anticancer Activity and Structural Characterization of Ubiquinones from Antrodia cinnamomea Mycelium. Molecules 2017, 22, 747.

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