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Molecules 2017, 22(5), 735; doi:10.3390/molecules22050735

Characterization of Chinese Unifloral Honeys Based on Proline and Phenolic Content as Markers of Botanical Origin, Using Multivariate Analysis

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1,2,3,4,* and 1,2,3,4,*
1
Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China
2
Bee Product Quality Supervision and Testing Center, Ministry of Agriculture, Beijing 100093, China
3
Laboratory of Risk Assessment for Quality and Safety of Bee Products, Ministry of Agriculture, Beijing 100093, China
4
Key Laboratory of Bee Products for Quality and Safety Control, Ministry of Agriculture, Beijing 100093, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 5 April 2017 / Revised: 27 April 2017 / Accepted: 28 April 2017 / Published: 17 May 2017
View Full-Text   |   Download PDF [2562 KB, uploaded 17 May 2017]   |  

Abstract

The phenolic and proline content were determined in honey samples of different floral origins (rapeseed, sunflower, buckwheat and Codonopsis) from five different regions of China. The phenolic and proline profile of these samples were used to construct a statistical model to distinguish honeys from different floral origins. Significant differences were identified among the studied honey samples from multivariate chemometric methods. The proline content varied among the four types of honeys, with the values decreasing in the order: buckwheat > Codonopsis > sunflower > rapeseed. Rapeseed honeys contained a high level of benzoic acid, while rutin, p-coumaric acid, p-hydroxybenzoic acid were present at relatively high levels in buckwheat honeys. Principal component analysis (PCA) revealed that rapeseed honey could be distinguished from the other three unifloral honeys, and benzoic acid, proline and kaempferol could serve as potential floral markers. Using 18 phenolic compounds and proline the honey samples were satisfactorily classified according to floral origin at 94% correct prediction by linear discriminant analysis (LDA). The results indicated that phenolic compounds and proline were useful for the identification of the floral origin of the four type honeys. View Full-Text
Keywords: botanical origin; phenolic compounds; proline; chemometrics; unifloral honeys botanical origin; phenolic compounds; proline; chemometrics; unifloral honeys
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Wen, Y.-Q.; Zhang, J.; Li, Y.; Chen, L.; Zhao, W.; Zhou, J.; Jin, Y. Characterization of Chinese Unifloral Honeys Based on Proline and Phenolic Content as Markers of Botanical Origin, Using Multivariate Analysis. Molecules 2017, 22, 735.

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