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Molecules 2017, 22(4), 602; doi:10.3390/molecules22040602

Screening, Purification and Characterization of Anionic Antimicrobial Proteins from Foeniculum Vulgare

1
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11451, Saudi Arabia
2
Department of Chemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
*
Authors to whom correspondence should be addressed.
Academic Editors: Paula A. C. Gomes and Stefania Galdiero
Received: 2 March 2017 / Revised: 5 April 2017 / Accepted: 6 April 2017 / Published: 8 April 2017
(This article belongs to the Special Issue Peptide-Based Drugs and Drug Delivery Systems)
View Full-Text   |   Download PDF [1058 KB, uploaded 11 April 2017]   |  

Abstract

Foeniculum vulgare Mill., commonly called fennel, is a medicinal plant belonging to the Umbelliferae (Apiaceae) family, and is used in traditional medicine. Antibacterial peptides were isolated using sodium phosphate citrate buffer and, for extraction, cetyltrimethyl ammonium bromide (CTAB) buffer with pH 6, have been employed and antimicrobial activity tested against four reference strains. The extracted protein was subjected to 3 kDa dialysis and separation was carried out by DEAE-ion exchange chromatography and further proteins were identified by 2D gel electrophoresis. The results of Foeniculum vulgare elutes obtained from DEAE-ion exchange chromatography were tested for antibacterial activity. Elute 3 shows the highest antibacterial activity on Pseudomonas aeruginosa with a diameter of a zone of inhibition of 16 mm and IC50 value 25.02 (mcg/mL). Based on the findings of the wide usage in treatment of various ailments and day-to-day life, Foeniculum vulgare seeds were used in the present research and have shown promising antibacterial effects, which requires further proteomic research to authenticate the role of the anticipated proteins. View Full-Text
Keywords: Foeniculum vulgare; ion exchange chromatography; antimicrobial proteins Foeniculum vulgare; ion exchange chromatography; antimicrobial proteins
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MDPI and ACS Style

Al Akeel, R.; Mateen, A.; Syed, R.; Alyousef, A.A.; Shaik, M.R. Screening, Purification and Characterization of Anionic Antimicrobial Proteins from Foeniculum Vulgare. Molecules 2017, 22, 602.

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