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Molecules 2017, 22(3), 418; doi:10.3390/molecules22030418

A Focused Multiple Reaction Monitoring (MRM) Quantitative Method for Bioactive Grapevine Stilbenes by Ultra-High-Performance Liquid Chromatography Coupled to Triple-Quadrupole Mass Spectrometry (UHPLC-QqQ)

1
Departamento Agroquímica y Bioquímica, Facultad de Ciencias, Universidad de Alicante, 03690 Alicante, Spain
2
Genomics and Proteomics Unit, SSTTI Universidad de Alicante, 03690 Alicante, Spain
3
Instituto de Investigación Sanitaria y Biomédica de Alicante ISABIAL-Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana FISABIO, 03010 Alicante, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Philippe Jeandet
Received: 23 January 2017 / Revised: 1 March 2017 / Accepted: 1 March 2017 / Published: 7 March 2017
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Abstract

Grapevine stilbenes are a family of polyphenols which derive from trans-resveratrol having antifungal and antimicrobial properties, thus being considered as phytoalexins. In addition to their diverse bioactive properties in animal models, they highlight a strong potential in human health maintenance and promotion. Due to this relevance, highly-specific qualitative and quantitative methods of analysis are necessary to accurately analyze stilbenes in different matrices derived from grapevine. Here, we developed a rapid, sensitive, and specific analysis method using ultra-high-performance liquid chromatography coupled to triple-quadrupole mass spectrometry (UHPLC-QqQ) in MRM mode to detect and quantify five grapevine stilbenes, trans-resveratrol, trans-piceid, trans-piceatannol, trans-pterostilbene, and trans-ε-viniferin, whose interest in relation to human health is continuously growing. The method was optimized to minimize in-source fragmentation of piceid and to avoid co-elution of cis-piceid and trans-resveratrol, as both are detected with resveratrol transitions. The applicability of the developed method of stilbene analysis was tested successfully in different complex matrices including cellular extracts of Vitis vinifera cell cultures, reaction media of biotransformation assays, and red wine. View Full-Text
Keywords: UHPLC; MRM; resveratrol; piceid; piceatannol; epsilon-viniferin; pterostilbene; cell culture; bioconversion; wine UHPLC; MRM; resveratrol; piceid; piceatannol; epsilon-viniferin; pterostilbene; cell culture; bioconversion; wine
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Hurtado-Gaitán, E.; Sellés-Marchart, S.; Martínez-Márquez, A.; Samper-Herrero, A.; Bru-Martínez, R. A Focused Multiple Reaction Monitoring (MRM) Quantitative Method for Bioactive Grapevine Stilbenes by Ultra-High-Performance Liquid Chromatography Coupled to Triple-Quadrupole Mass Spectrometry (UHPLC-QqQ). Molecules 2017, 22, 418.

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