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Molecules 2017, 22(3), 416; doi:10.3390/molecules22030416

Simultaneous Determination of Three Furanocoumarins by UPLC/MS/MS: Application to Pharmacokinetic Study of Angelica dahurica Radix after Oral Administration to Normal and Experimental Colitis-Induced Rats

KM Application Center, Korea Institute of Oriental Medicine, Daegu 41062, Korea
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Academic Editor: Derek J. McPhee
Received: 9 January 2017 / Revised: 21 February 2017 / Accepted: 27 February 2017 / Published: 7 March 2017
(This article belongs to the Section Natural Products)
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Abstract

In traditional oriental medicine, Angelica dahurica Radix (ADR) is used in the treatment of gastrointestinal, respiratory, neuromuscular, and dermal disorders. We evaluated the pharmacokinetic profiles of oxypeucedanin, imperatorin, and isoimperatorin, major active ingredients of ADR, in normal and 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis rats. A rapid, sensitive, and validated UPLC/MS/MS method was established for evaluating the pharmacokinetics of three furanocoumarins. After oral administration of ADR (0.5 and 1.0 g/kg), blood samples were collected periodically from the tail vein. In colitis rats, the time to reach the peak concentration (Tmax) of imperatorin and isoimperatorin was significantly delayed (p < 0.05). Lower peak plasma concentrations (Cmax) and longer mean residence times for all furanocoumarins were also observed (p < 0.05) compared with normal rats. There was no significant difference in the area under the plasma concentration–time curve or elimination half-lives. Thus, the delayed Tmax and decreased Cmax, with no influence on the elimination half-life, could be colitis-related changes in the drug-absorption phase. Therefore, the prescription and use of ADR in colitis patients should receive more attention. View Full-Text
Keywords: Angelica dahurica Radix; imperatorin; isoimperatorin; oxypeucedanin; pharmacokinetics; colitis Angelica dahurica Radix; imperatorin; isoimperatorin; oxypeucedanin; pharmacokinetics; colitis
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Hwang, Y.-H.; Yang, H.J.; Ma, J.Y. Simultaneous Determination of Three Furanocoumarins by UPLC/MS/MS: Application to Pharmacokinetic Study of Angelica dahurica Radix after Oral Administration to Normal and Experimental Colitis-Induced Rats. Molecules 2017, 22, 416.

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