Next Article in Journal
Peptide-Based Drugs and Drug Delivery Systems
Previous Article in Journal
In Vitro Evaluation of Cytotoxicity and Permeation Study on Lysine- and Arginine-Based Lipopeptides with Proven Antimicrobial Activity
Article Menu
Issue 12 (December) cover image

Export Article

Open AccessArticle
Molecules 2017, 22(12), 2175; doi:10.3390/molecules22122175

Preparation of β-CD-Ellagic Acid Microspheres and Their Effects on HepG2 Cell Proliferation

1
College of Life Science, Zaozhuang University, Zaozhuang 277160, China
2
College of Medical Science, Zaozhuang Vocational College, Zaozhuang 277800, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 13 October 2017 / Revised: 29 November 2017 / Accepted: 6 December 2017 / Published: 8 December 2017
(This article belongs to the Section Medicinal Chemistry)
View Full-Text   |   Download PDF [3746 KB, uploaded 8 December 2017]   |  

Abstract

Objective: In this study, β-cyclodextrin (β-CD) was chosen as the coating for ellagic acid to prepare ellagic acid microspheres, and the effect of microspheres on the growth of HepG2 cells was observed. Methods: Scanning electron microscopy, infrared spectroscopy, and release rate analysis were used to identify the formation of ellagic acid microspheres. Methyl thiazolyl tetrazolium (MTT) assay was used to detect the effect of different concentrations of ellagic acid microspheres on tumor cell proliferation at 6, 12, 24 and 36 h, and cell morphology and quantity were observed using hematoxylin-eosin (HE) staining. Single-cell gel electrophoresis was used to observe the effect of ellagic acid microspheres on the DNA damage of HepG2 cells, and the Olive tail moment and the mRNA expression of tumor suppressor protein gene p53 was measured. Results: β-CD could be used as wrapping material of ellagic acid to prepare ellagic acid microspheres. HepG2 cell proliferation could be inhibited by 0.1, 0.3 and 0.5 g/L of ellagic acid microspheres in a dose- and time-dependent manner, and the mechanism of proliferation inhibition was related to DNA damage and cell apoptosis. Conclusion: Preparing ellagic acid microspheres with β-CD is feasible, and ellagic acid microspheres have potential therapeutic value (anticancer). View Full-Text
Keywords: β-CD-ellagic acid microspheres; preparation; identification; HepG2 cell proliferations β-CD-ellagic acid microspheres; preparation; identification; HepG2 cell proliferations
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Wang, H.; Zhang, Y.; Tian, Z.; Ma, J.; Kang, M.; Ding, C.; Ming, D. Preparation of β-CD-Ellagic Acid Microspheres and Their Effects on HepG2 Cell Proliferation. Molecules 2017, 22, 2175.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top