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Molecules 2017, 22(12), 2172; https://doi.org/10.3390/molecules22122172

Extraction Optimization of Flavonoids from Hypericum formosanum and Matrix Metalloproteinase-1 Inhibitory Activity

1
Department of Food Science, National Pingtung University of Science & Technology, Pingtung 91201, Taiwan
2
Testing Center of Cosmetic, Tridl Inc., Tainan 70955, Taiwan
*
Author to whom correspondence should be addressed.
Received: 17 October 2017 / Revised: 25 November 2017 / Accepted: 2 December 2017 / Published: 11 December 2017
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Abstract

Hypericum formosanum is a valuable herb in Taiwan. In this study, response surface methodology was employed to optimize the ultrasound-assisted extraction of flavonoids from Hypericum formosanum. A central composite design with three variables (ethanol concentration, extraction time, and extraction temperature) was applied. Experimental results were fitted to the second order polynomial model and one-way analysis of variance was used to determine the goodness of fit of the model and the optimal conditions for responses. The optimal conditions for the maximum extraction yield of total flavonoid content (101.1 mg/g) using ultrasound-assisted extraction were ethanol concentration, 73.5%; extraction time, 38.3 min; and extraction temperature, 62.5 °C. The predicted result was consistent with the experimental result obtained under optimal extraction conditions. Hyperoside, astilbin, quercitrin, and quercetin from Hypericum formosanum extract (HFE) were identified by Ultra performance liquid chromatography-diode array detector-mass (UPLC-DAD-MS). HFE significantly reduced matrix metalloproteinase-1 protein expression in human skin keratinocyte cells, induced by advanced glycation end products. View Full-Text
Keywords: response surface methodology; Hypericum formosanum; matrix metalloproteinase-1; flavonoids response surface methodology; Hypericum formosanum; matrix metalloproteinase-1; flavonoids
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Huang, H.-S.; Liaw, E.-T. Extraction Optimization of Flavonoids from Hypericum formosanum and Matrix Metalloproteinase-1 Inhibitory Activity. Molecules 2017, 22, 2172.

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