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Molecules 2017, 22(12), 2110; https://doi.org/10.3390/molecules22122110

Metabolomics Strategy Using High Resolution Mass Spectrometry Reveals Novel Biomarkers and Pain-Relief Effect of Traditional Chinese Medicine Prescription Wu-Zhu-Yu Decoction Acting on Headache Modelling Rats

1
National and Local Joint Engineering Laboratory for Key Technology of Chinese Material Medica Quality Control, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Rd., Shenyang 110016, China
2
Guangzhou Bristol Drug Delivery Co., Ltd., 11 Kaiyuan Ave, Guangzhou 510320, China
3
China Food and Drug Administration Institute of Executive Development, 16 Xi Zhan Nan Rd., Beijing 100073, China
4
Dalian Institute for Drug Control, 888a Huanghe Rd., Dalian 116000, China
*
Author to whom correspondence should be addressed.
Received: 23 October 2017 / Revised: 26 November 2017 / Accepted: 30 November 2017 / Published: 18 December 2017
(This article belongs to the Section Natural Products Chemistry)
View Full-Text   |   Download PDF [3085 KB, uploaded 18 December 2017]   |  

Abstract

Headache is a common episodic or chronic neurologic disorder. Treatment options and diagnosis are restricted by an incomplete understanding of disease pathology and the lack of diagnostic markers. Wu-Zhu-Yu decoction (WZYD), a traditional Chinese medicine (TCM) formula containing four TCM herbs, is commonly used in the treatment of headache in China. To deeply understand more about headache and investigate the pain-relief mechanism of WZYD, a comprehensive metabolomics study combined with multivariate data processing strategy was carried out. An LC-high resolution mass spectrometry-based metabolomics approach was applied to characterize metabolic biomarker candidates. Multiple pattern recognition including principal component analysis-discriminant analysis, partial least squares-discriminant analysis and hierarchical cluster analysis were used to determine groups and confirm important variables. A total of 17 potential biomarkers were characterized and related metabolic pathways were identified. The study demonstrated that the established metabolomics strategy is a powerful approach for investigating the mechanism of headache attack and WZYD. In addition, the approach may highlight biomarkers and metabolic pathways and can capture subtle metabolite changes from headache, which may lead to an improved mechanism understanding of central nervous system diseases and TCM treatment. View Full-Text
Keywords: headache; high resolution mass spectrometry; metabolomics; traditional Chinese medicine; Wu-Zhu-Yu decoction headache; high resolution mass spectrometry; metabolomics; traditional Chinese medicine; Wu-Zhu-Yu decoction
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Liu, R.; Xu, H.; Zhang, X.; Wang, X.; Yuan, Z.; Sui, Z.; Wang, D.; Bi, K.; Li, Q. Metabolomics Strategy Using High Resolution Mass Spectrometry Reveals Novel Biomarkers and Pain-Relief Effect of Traditional Chinese Medicine Prescription Wu-Zhu-Yu Decoction Acting on Headache Modelling Rats. Molecules 2017, 22, 2110.

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