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Molecules 2017, 22(11), 1918; doi:10.3390/molecules22111918

Effects of Polysaccharides from Platycodon grandiflorum on Immunity-Enhancing Activity In Vitro

1
College of Animal Medicine and Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, China
2
Research Center for Animal Disease Control Engineering Shandong Province, Shandong Agricultural University, Tai’an 271018, China
3
Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, 61 Daizong Street, Tai’an 271018, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 28 September 2017 / Revised: 30 October 2017 / Accepted: 2 November 2017 / Published: 7 November 2017
(This article belongs to the Special Issue Polysaccharide-based Materials)
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Abstract

The study is aimed at investigating the immunoenhancement activity of polysaccharides from Platycodon grandiflorum polysaccharides (PGPSs) in vitro. In this study, some research on lymphocyte proliferation, cell cycle, and the levels of CD4+ and CD8+ T cells were performed. Four different concentrations of PGPSs (PGPStc, PGPS60c, PGPS80c, and PGPStp) were harvested and added to peripheral blood T lymphocytes. We observed significant increases in T lymphocyte proliferation at PGPStc groups individually or synergistically with phytohemagglutinin (PHA) at most concentrations, and their lymphocyte proliferation rates were the highest. The active sites of PGPStc and PGPS60c were subsequently chosen. Then, we utilized flow cytometry to determine lymphocyte cell cycle distribution and levels of CD4+ and CD8+ T cells. At most time points, PGPStc could facilitate lymphocyte cell cycle progression from the G0/G1 phase to the S and G2/M phases and, simultaneously, increase the levels of CD4+ and CD8+ T cells. These results indicate that PGPStc enhances the immune functions, suggesting that PGPStc could be a potential immunopotentiator for further in vivo and clinical trial experiments. View Full-Text
Keywords: Platycodon grandiflorum; polysaccharides; lymphocyte proliferation; cell cycle; immunity-enhancing Platycodon grandiflorum; polysaccharides; lymphocyte proliferation; cell cycle; immunity-enhancing
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Zhao, X.; Wang, Y.; Yan, P.; Cheng, G.; Wang, C.; Geng, N.; Wang, X.; Liu, J. Effects of Polysaccharides from Platycodon grandiflorum on Immunity-Enhancing Activity In Vitro. Molecules 2017, 22, 1918.

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