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Molecules 2017, 22(11), 1853; doi:10.3390/molecules22111853

Simultaneous Determination of Twenty-Five Compounds in Rat Plasma Using Ultra-High Performance Liquid Chromatography-Polarity Switching Tandem Mass Spectrometry and Its Application to a Pharmacokinetic Study

1
Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China
2
Department of pharmacy, Baotou Medical College, Baotou 014060, China
These authors contributed equally to this article.
*
Author to whom correspondence should be addressed.
Received: 13 October 2017 / Revised: 25 October 2017 / Accepted: 27 October 2017 / Published: 30 October 2017
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [1367 KB, uploaded 31 October 2017]   |  

Abstract

An attempt was made to characterize the pharmacokinetic profiles of Qishen Keli (QSKL) that has been widely proved to be effective in clinical practice. A method using ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) for the simultaneous determination of 25 analytes in rat plasma was developed and validated. Satisfactory chromatographic separation was achieved on an ACQUITY UPLC HSS T3 column with gradient elution using mobile phase consisting of 0.02% aqueous formic acid (A) and acetonitrile fortified with 0.02% formic acid (B), and analyte detection was carried out using polarity-switching multiple reaction monitoring mode. Method validation assays in terms of selectivity, linearity, inter- and intra-day variations, matrix effect, and recovery demonstrated the newly developed method to be specific, sensitive, accurate, and precise. Following the oral administration of QSKL at a single dose, the qualified method was successfully applied for pharmacokinetic investigations in sham and model rats. Mild differences occurred for the pharmacokinetic patterns of most components between those two groups, whereas significant differences were observed for glycyrrhizic acid and glycyrrhetic acid. The obtained findings could provide meaningful information for the clarification of the effective material basis of QSKL. View Full-Text
Keywords: Qishen Keli; multi-component pharmacokinetics; polarity-switching multiple reaction monitoring; simultaneous determination; ultra-high performance liquid chromatography Qishen Keli; multi-component pharmacokinetics; polarity-switching multiple reaction monitoring; simultaneous determination; ultra-high performance liquid chromatography
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Zhang, N.; Li, Y.; Sun, J.; Li, C.; Song, Y.; Li, J.; Tu, P.; Zhao, Y. Simultaneous Determination of Twenty-Five Compounds in Rat Plasma Using Ultra-High Performance Liquid Chromatography-Polarity Switching Tandem Mass Spectrometry and Its Application to a Pharmacokinetic Study. Molecules 2017, 22, 1853.

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