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Molecules 2017, 22(10), 1807; doi:10.3390/molecules22101807

Design of Novel, Water Soluble and Highly Luminescent Europium Labels with Potential to Enhance Immunoassay Sensitivities

1
Radiometer Turku Oy, Biolinja 12, 20750 Turku, Finland
2
Present affiliation ArcDia International Oy Ltd, Lemminkäisenkatu 32, 20520 Turku, Finland
*
Author to whom correspondence should be addressed.
Received: 29 September 2017 / Revised: 17 October 2017 / Accepted: 19 October 2017 / Published: 24 October 2017
(This article belongs to the Special Issue Lanthanide Luminescence: Fundamental Research and Applications)
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Abstract

To meet the continual demands of more-sensitive immunoassays, the synthesis of novel luminescent Eu(III) chelate labels having similar substituted 4-(phenylethynyl)pyridine chromophores in three different chelate structure classes are reported. Significantly enhanced luminescence intensities were obtained, evidently caused by the intra-ligand charge transfer (ILCT) mediated sensitization, but the alternative ligands triplet state process cannot be ruled out. Based on the present study, even quite small changes on the chelate structure, and, especially, on the substituents’ donor/acceptor strength on both ends of 4-(phenylethynyl)pyridine subunits have an unpredictable effect on the luminescence. The highest observed brightness was 16,400 M−1cm−1 in solution and 69,500 M−1cm−1 on dry surface, being 3.4 and 8.7 fold higher compared to the reference chelate. The new label chelates provide solutions for improved assay sensitivity up-to tenfold from the present concepts. View Full-Text
Keywords: europium chelate; label; (phenylethynyl)pyridine chromophore; luminescence; intra-ligand charge transfer europium chelate; label; (phenylethynyl)pyridine chromophore; luminescence; intra-ligand charge transfer
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MDPI and ACS Style

Sund, H.; Blomberg, K.; Meltola, N.; Takalo, H. Design of Novel, Water Soluble and Highly Luminescent Europium Labels with Potential to Enhance Immunoassay Sensitivities. Molecules 2017, 22, 1807.

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