α-Synuclein Regulates Neuronal Cholesterol Efflux
Abstractα-Synuclein is a neuronal protein that is at the center of focus in understanding the etiology of a group of neurodegenerative diseases called α-synucleinopathies, which includes Parkinson’s disease (PD). Despite much research, the exact physiological function of α-synuclein is still unclear. α-Synuclein has similar biophysical properties as apolipoproteins and other lipid-binding proteins and has a high affinity for cholesterol. These properties suggest a possible role for α-synuclein as a lipid acceptor mediating cholesterol efflux (the process of removing cholesterol out of cells). To test this concept, we “loaded” SK-N-SH neuronal cells with fluorescently-labelled cholesterol, applied exogenous α-synuclein, and measured the amount of cholesterol removed from the cells using a classic cholesterol efflux assay. We found that α-synuclein potently stimulated cholesterol efflux. We found that the process was dose and time dependent, and was saturable at 1.0 µg/mL of α-synuclein. It was also dependent on the transporter protein ABCA1 located on the plasma membrane. We reveal for the first time a novel role of α-synuclein that underscores its importance in neuronal cholesterol regulation, and identify novel therapeutic targets for controlling cellular cholesterol levels. View Full-Text
Share & Cite This Article
Hsiao, J.-H.T.; Halliday, G.M.; Kim, W.S. α-Synuclein Regulates Neuronal Cholesterol Efflux. Molecules 2017, 22, 1769.
Hsiao J-HT, Halliday GM, Kim WS. α-Synuclein Regulates Neuronal Cholesterol Efflux. Molecules. 2017; 22(10):1769.Chicago/Turabian Style
Hsiao, Jen-Hsiang T.; Halliday, Glenda M.; Kim, Woojin S. 2017. "α-Synuclein Regulates Neuronal Cholesterol Efflux." Molecules 22, no. 10: 1769.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.