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Molecules 2017, 22(10), 1637; doi:10.3390/molecules22101637

Fabrication of Antimicrobial Peptide-Loaded PLGA/Chitosan Composite Microspheres for Long-Acting Bacterial Resistance

1
State Key Laboratory of New Ceramic & Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China
2
Department of Prosthodontics, School of Stomatology, Dalian Medical University, Dalian 116044, China
3
Department of Stomatology, Shengli Oil Field Central Hospital, Dongying 257034, China
*
Authors to whom correspondence should be addressed.
Received: 14 September 2017 / Revised: 23 September 2017 / Accepted: 25 September 2017 / Published: 29 September 2017
(This article belongs to the Special Issue Biomedical Applications of Polylactide (PLA) and its Copolymers)
View Full-Text   |   Download PDF [4693 KB, uploaded 29 September 2017]   |  

Abstract

An antimicrobial decapeptide, KSL-W (KKVVFWVKFK-CONH2), which could maintain stable antimicrobial activity in saliva, has therefore been widely used to inhibit biofilm formation on teeth and prevent the growth of oral microorganisms for related infectious diseases treatment. In order to control the release of KSL-W for long-term bacterial resistance, KSL-W-loaded PLGA/chitosan composite microspheres (KSL/PLGA/CS MSs) were prepared by electrospraying and combined crosslinking-emulsion methods. Different formulations of microspheres were characterized as to surface morphology, size distribution, encapsulation efficiency, in vitro drug release, and antimicrobial activity. Antibacterial experiment demonstrated the prolonged antimicrobial and inhibitory effects of KSL/PLGA/CS MSs on oral bacteria. Moreover, the cell proliferation assay proved that the released KSL-W antibacterial dosage had no cytotoxicity to the growth of osteoblast MC3T3-E1. Thus, our study suggested that the KSL-W-loaded PLGA/CS composite microspheres may have potentially therapeutic applications as an effective drug delivery system in the treatment of oral infectious diseases such as periodontitis and periodontitis, and also within bone graft substitutes for alveolar bone augmentation. View Full-Text
Keywords: antimicrobial peptides; electrospraying; bacterial resistance; PLGA; composite microspheres antimicrobial peptides; electrospraying; bacterial resistance; PLGA; composite microspheres
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Li, Y.; Na, R.; Wang, X.; Liu, H.; Zhao, L.; Sun, X.; Ma, G.; Cui, F. Fabrication of Antimicrobial Peptide-Loaded PLGA/Chitosan Composite Microspheres for Long-Acting Bacterial Resistance. Molecules 2017, 22, 1637.

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