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Molecules 2017, 22(10), 1618; doi:10.3390/molecules22101618

A Fluorescent Coumarin-Based Probe for the Fast Detection of Cysteine with Live Cell Application

School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
Experiment Center of Teaching & Learning, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
These authors contributed equally to this work.
Authors to whom correspondence should be addressed.
Received: 23 August 2017 / Revised: 19 September 2017 / Accepted: 20 September 2017 / Published: 26 September 2017
View Full-Text   |   Download PDF [3405 KB, uploaded 26 September 2017]   |  


A new coumarin-based fluorescent probe, containing an allylic esters group, has been designed and synthesized for sensing cysteine in physiological pH. In this fluorescent probe, the coumarin was applied as the fluorophore and an allylic esters group was combined as both a fluorescence quencher and a recognition unit. The probe can selectively and sensitively detect cysteine (Cys) over homocysteine, glutathione, and other amino acids, and has a rapid response time of 30 min and a low detection limit of 47.7 nM. In addition, the probe could be applied for cell imaging with low cytotoxicity. View Full-Text
Keywords: cysteine; fluorescent probe; coumarin; bioimaging cysteine; fluorescent probe; coumarin; bioimaging

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Zeng, R.-F.; Lan, J.-S.; Li, X.-D.; Liang, H.-F.; Liao, Y.; Lu, Y.-J.; Zhang, T.; Ding, Y. A Fluorescent Coumarin-Based Probe for the Fast Detection of Cysteine with Live Cell Application. Molecules 2017, 22, 1618.

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