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Molecules 2017, 22(1), 174; doi:10.3390/molecules22010174

A Method for LC-MS/MS Profiling of Coumarins in Zanthoxylum zanthoxyloides (Lam.) B. Zepernich and Timler Extracts and Essential Oils

1
Université de Corse, UMR CNRS 6134 SPE, Laboratoire de Chimie des Produits Naturels, Campus Grimaldi, BP 52, F-20250 Corte, France
2
Laboratoire de Chimie Organique et Thérapeutique, Faculté de Médecine, Pharmacie et Odontologie, Université Cheikh Anta Diop, BP 5005 Dakar-Fann, Senegal
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 18 November 2016 / Revised: 26 December 2016 / Accepted: 9 January 2017 / Published: 22 January 2017
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Abstract

The metabolites from the coumarin class, present in tissues of plants belonging mainly to the Rutaceae and Apiaceae families, included compounds with high chemical diversity such as simple coumarins and furocoumarins. These health-promoting components are recognized for their valuable biological activities in herbal preparations but also for their phototoxic effects. In this work, a targeted liquid chromatography (LC) coupled with tandem mass spectrometry (MS2) was developed for the screening of 39 reference standards of coumarins and furocoumarins in essential oils and plant extracts. Chromatographic separation was accomplished on reversed phase column using water/acetonitrile as the mobile phase and detection was performed on a hybrid QqQ/linear ion trap spectrometer fitted with an atmospheric pressure chemical ionization (APCI) source operating in positive ion mode. This analytical approach was applied to investigate the coumarin compositions of fruit essential oils and methanolic extracts obtained from separated parts (fruit, leaf, stem, trunk, and root) of Zanthoxylum zanthoxyloides. Ten coumarins and six furanocoumarins were reported in this species and data analyses were used to assess the suitability of these compounds to the metabolomics-based differentiation of plant organs. The quantification criteria of the metabolites in extract samples included linearity, limit of quantification, limit of detection, and matrix effect were validated. As reported for other species of the Rutaceae family, the concentration of coumarins was drastically higher in Z. zanthoxyloides fruits than in other plant organs. View Full-Text
Keywords: essential oil; coumarins; Zanthoxylum zanthoxyloides; LC-APCI-MS/MS essential oil; coumarins; Zanthoxylum zanthoxyloides; LC-APCI-MS/MS
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Tine, Y.; Renucci, F.; Costa, J.; Wélé, A.; Paolini, J. A Method for LC-MS/MS Profiling of Coumarins in Zanthoxylum zanthoxyloides (Lam.) B. Zepernich and Timler Extracts and Essential Oils. Molecules 2017, 22, 174.

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