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Erratum published on 27 March 2017, see Molecules 2017, 22(4), 534.

Open AccessArticle
Molecules 2017, 22(1), 123; doi:10.3390/molecules22010123

Novel Antihypertensive Peptides Derived from Adlay (Coix larchryma-jobi L. var. ma-yuen Stapf) Glutelin

Beijing University of Chinese Medicine, 6 South Zhonghuan Road, Wangjing, Chaoyang District, Beijing 100102, China
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Authors to whom correspondence should be addressed.
Academic Editor: Diego Muñoz-Torrero
Received: 9 October 2016 / Revised: 25 November 2016 / Accepted: 29 December 2016 / Published: 13 January 2017
(This article belongs to the Special Issue Peptide-Based Drugs and Drug Delivery Systems)
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Abstract

Our previous studies have shown that Coix glutelin pepsin hydrolysate can effectively inhibit angiotensin converting enzyme (ACE) activity in vitro. The main purpose of this study was to obtain potent anti-hypertensive peptides from Coix glutelin. The Coix glutelin hydrolysates (CGH) were prepared by pepsin catalysis and further separated by an ultrafitration (UF) system, gel filtration chromatography (GFC) and reversed-phase high performance liquid chromatography (RP-HPLC). As a result, the sub-fraction F5-3 had the highest ACE-inhibitory activity. Six ACE inhibitory peptides were identified using nano-liquid chromatography coupled to tandem mass spectrometry. The most potent peptide GAAGGAF (IC50 = 14.19 μmol·L−1) was finally obtained by further molecular simulation screening and a series of division and optimization. Single oral administration of synthesized GAAGGAF at 15 mg/kg body weight (BW) in spontaneously hypertensive rats (SHR) could reduce the systolic blood pressure (SBP) around 27.50 mmHg and the effect lasted for at least 8 h. The study demonstrated for the first time that the ACE inhibitory peptide GAAGGAF from Coix glutelin has a significant antihypertensive effect, and it could be a good natural ingredient for pharmaceuticals against hypertension and the related diseases. View Full-Text
Keywords: Coix larchryma-jobi L. var. ma-yuen Stapf; glutelin; angiotensin I-converting enzyme; antihypertensive peptide; molecular simulation Coix larchryma-jobi L. var. ma-yuen Stapf; glutelin; angiotensin I-converting enzyme; antihypertensive peptide; molecular simulation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Li, B.; Qiao, L.; Li, L.; Zhang, Y.; Li, K.; Wang, L.; Qiao, Y. Novel Antihypertensive Peptides Derived from Adlay (Coix larchryma-jobi L. var. ma-yuen Stapf) Glutelin. Molecules 2017, 22, 123.

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