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Molecules 2016, 21(9), 1143; doi:10.3390/molecules21091143

In Vivo Targeted MR Imaging of Endogenous Neural Stem Cells in Ischemic Stroke

Department of Radiology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, Guangdong, China
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Author to whom correspondence should be addressed.
Academic Editor: Zhen Cheng
Received: 20 July 2016 / Revised: 19 August 2016 / Accepted: 26 August 2016 / Published: 29 August 2016
(This article belongs to the Special Issue Molecular Imaging Probes)
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Abstract

Acute ischemic stroke remains a leading cause of death and disability. Endogenous neurogenesis enhanced via activation of neural stem cells (NSCs) could be a promising method for stroke treatment. In vivo targeted tracking is highly desirable for monitoring the dynamics of endogenous NSCs in stroke. Previously, we have successfully realized in vivo targeted MR imaging of endogenous NSCs in normal adult mice brains by using anti-CD15 antibody-conjugated superparamagnetic iron oxide nanoparticles (anti-CD15-SPIONs) as the molecular probe. Herein, we explore the performance of this molecular probe in targeted in vivo tracking of activated endogenous NSCs in ischemic stroke. Our study showed that intraventricular injection of anti-CD15-SPIONs could label activated endogenous NSCs in situ seven days after ischemic stroke, which were detected as enlarged areas of hypo-intense signals on MR imaging at 7.0 T. The treatment of cytosine arabinosine could inhibit the activation of endogenous NSCs, which was featured by the disappearance of areas of hypo-intense signals on MR imaging. Using anti-CD15-SPIONs as imaging probes, the dynamic process of activation of endogenous NSCs could be readily monitored by in vivo MR imaging. This targeted imaging strategy would be of great benefit to develop a new therapeutic strategy utilizing endogenous NSCs for ischemic stroke. View Full-Text
Keywords: neural stem cells; adult; ischemic stroke; magnetic resonance imaging; monoclonal anit-CD15 antibody; iron oxide nanoparticles neural stem cells; adult; ischemic stroke; magnetic resonance imaging; monoclonal anit-CD15 antibody; iron oxide nanoparticles
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Zhang, F.; Duan, X.; Lu, L.; Zhang, X.; Zhong, X.; Mao, J.; Chen, M.; Shen, J. In Vivo Targeted MR Imaging of Endogenous Neural Stem Cells in Ischemic Stroke. Molecules 2016, 21, 1143.

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