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Molecules 2016, 21(8), 985; doi:10.3390/molecules21080985

Influences of Adhesion Variability on the “Living” Dynamics of Filamentous Bacteria in Microfluidic Channels

1
U.S. Army Research Laboratory, Adelphi, MD 20783, USA
2
Department of Materials Science and Engineering, Bioengineering Program, Lehigh University, Bethlehem, PA 18015, USA
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editors: Fan-Gang Tseng and Tuhin Subhra Santra
Received: 20 May 2016 / Revised: 18 July 2016 / Accepted: 21 July 2016 / Published: 28 July 2016
(This article belongs to the Special Issue Micro/Nano Fluidics and Bio-MEMS)
View Full-Text   |   Download PDF [2088 KB, uploaded 28 July 2016]   |  

Abstract

Microfabricated devices have increasingly incorporated bacterial cells for microscale studies and exploiting cell-based functions in situ. However, the role of surface interactions in controlling the bacterial cell behavior is not well understood. In this study, microfluidic substrates of varied bacterial-binding affinity were used to probe the interaction-driven behavior of filamentous Escherichia coli. In particular, cell alignment under controlled shear flow as well as subsequent orientation and filamentation were compared between cells presenting distinct outer membrane phenotypes. We demonstrated that filaments retained position under flow, which allowed for dynamic single-cell monitoring with in situ elongation of over 100 μm for adherent cells. This maximum was not reached by planktonic cells and was, therefore, adhesion-dependent. The bound filaments initially aligned with flow under a range of flow rates and their continual elongation was traced in terms of length and growth path; analysis demonstrated that fimbriae-mediated adhesion increased growth rate, increased terminal length, as well as dramatically changed the adherent geometry, particularly buckling behavior. The effects to filament length and buckling were further exaggerated by the strongest, specificity-driven adhesion tested. Such surface-guided control of the elongation process may be valuable to yield interesting “living” filamentous structures in microdevices. In addition, this work may offer a biomedically relevant platform for further elucidation of filamentation as an immune-resistant morphology. Overall, this work should inspire broader exploration of microfabricated devices for the study and application of single bacterial cells. View Full-Text
Keywords: filamentous bacteria; fimbriae; mannosylated substrate; adhesion; orientation; microfluidic; buckle; shear flow filamentous bacteria; fimbriae; mannosylated substrate; adhesion; orientation; microfluidic; buckle; shear flow
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Jahnke, J.P.; Terrell, J.L.; Smith, A.M.; Cheng, X.; Stratis-Cullum, D.N. Influences of Adhesion Variability on the “Living” Dynamics of Filamentous Bacteria in Microfluidic Channels. Molecules 2016, 21, 985.

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