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Molecules 2016, 21(8), 1067; doi:10.3390/molecules21081067

Rapid Isolation and Determination of Flavones in Biological Samples Using Zinc Complexation Coupled with High-Performance Liquid Chromatography

1,2,†
,
1,2,†
,
1
and
1,2,*
1
Institute of Special Wild Animals and Plants, Chinese Academy of Agricultural Science, Changchun 130122, China
2
School of Life Science, Beijing Institute of Technology, Beijing 100081, China
These authors contributed equally to this study.
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 16 June 2016 / Revised: 2 August 2016 / Accepted: 9 August 2016 / Published: 16 August 2016
(This article belongs to the Special Issue Flavonoids: From Structure to Health Issues)
View Full-Text   |   Download PDF [1935 KB, uploaded 16 August 2016]   |  

Abstract

Chlorophyll-type contaminants are commonly encountered in the isolation and determination of flavones of plant aerial plant parts. Heme is also a difficult background substance in whole blood analysis. Both chlorophyll and heme are porphyrin type compounds. In this study, a rapid method for isolating flavones with 5-hydroxyl or ortho-hydroxyl groups from biological samples was developed based on the different solubilities of porphyrin-metal and flavone-metal complexes. It is important that other background substances, e.g., proteins and lipids, are also removed from flavones without an additional processing. The recoveries of scutellarin, baicalin, baicalein, wogonoside and wogonin, which are the primary constituents of Scutellaria baicalensis (skullcaps) were 99.65% ± 1.02%, 98.98% ± 0.73%, 99.65% ± 0.03%, 97.59% ± 0.09% and 95.19% ± 0.47%, respectively. As a sample pretreatment procedure, this method was coupled to high-performance liquid chromatography (HPLC) with good separation, sensitivity and linearity and was applied to determine the flavone content in different aerial parts of S. baicalensis and in dried blood spot samples. View Full-Text
Keywords: Scutellaria baicalensis; dried blood spot; metal complexation; high-performance liquid chromatography; chlorophyll; heme Scutellaria baicalensis; dried blood spot; metal complexation; high-performance liquid chromatography; chlorophyll; heme
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Sun, C.; Wang, H.; Wang, Y.; Xiao, S. Rapid Isolation and Determination of Flavones in Biological Samples Using Zinc Complexation Coupled with High-Performance Liquid Chromatography. Molecules 2016, 21, 1067.

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