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Molecules 2016, 21(7), 881; doi:10.3390/molecules21070881

Development of Droplet Microfluidics Enabling High-Throughput Single-Cell Analysis

1
Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China
2
Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
*
Authors to whom correspondence should be addressed.
Academic Editors: Fan-Gang Tseng and Tuhin Subhra Santra
Received: 30 May 2016 / Revised: 27 June 2016 / Accepted: 28 June 2016 / Published: 5 July 2016
(This article belongs to the Special Issue Micro/Nano Fluidics and Bio-MEMS)
View Full-Text   |   Download PDF [2480 KB, uploaded 5 July 2016]   |  

Abstract

This article reviews recent developments in droplet microfluidics enabling high-throughput single-cell analysis. Five key aspects in this field are included in this review: (1) prototype demonstration of single-cell encapsulation in microfluidic droplets; (2) technical improvements of single-cell encapsulation in microfluidic droplets; (3) microfluidic droplets enabling single-cell proteomic analysis; (4) microfluidic droplets enabling single-cell genomic analysis; and (5) integrated microfluidic droplet systems enabling single-cell screening. We examine the advantages and limitations of each technique and discuss future research opportunities by focusing on key performances of throughput, multifunctionality, and absolute quantification. View Full-Text
Keywords: droplet microfluidics; single-cell encapsulation; single-cell proteomic analysis; single-cell genetic analysis; single-cell screening; high-throughput droplet microfluidics; single-cell encapsulation; single-cell proteomic analysis; single-cell genetic analysis; single-cell screening; high-throughput
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Wen, N.; Zhao, Z.; Fan, B.; Chen, D.; Men, D.; Wang, J.; Chen, J. Development of Droplet Microfluidics Enabling High-Throughput Single-Cell Analysis. Molecules 2016, 21, 881.

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