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Molecules 2016, 21(7), 831; doi:10.3390/molecules21070831

A Metagenomic Advance for the Cloning and Characterization of a Cellulase from Red Rice Crop Residues

1
Departament of Biology and Graduate Program in Agricultural Sciences, State University of Paraíba–UEPB, Campina Grande–PB 58429-500, Brazil
2
Graduate Program in Agricultural Sciences, State University of Paraíba–UEPB, Campina Grande–PB 58429-500, Brazil
3
Departament of Biology, State University of Paraíba–UEPB, Campina Grande–PB 58429-500, Brazil
4
Department of Biochemistry and Molecular Biology, Federal University of Paraná–UFPR, Curitiba–PR 81.531-980, Brazil
5
Indigo Agriculture, Boston, MA 02129, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 1 June 2016 / Revised: 20 June 2016 / Accepted: 22 June 2016 / Published: 25 June 2016
(This article belongs to the Section Molecular Diversity)
View Full-Text   |   Download PDF [2621 KB, uploaded 25 June 2016]   |  

Abstract

Many naturally-occurring cellulolytic microorganisms are not readily cultivable, demanding a culture-independent approach in order to study their cellulolytic genes. Metagenomics involves the isolation of DNA from environmental sources and can be used to identify enzymes with biotechnological potential from uncultured microbes. In this study, a gene encoding an endoglucanase was cloned from red rice crop residues using a metagenomic strategy. The amino acid identity between this gene and its closest published counterparts is lower than 70%. The endoglucanase was named EglaRR01 and was biochemically characterized. This recombinant protein showed activity on carboxymethylcellulose, indicating that EglaRR01 is an endoactive lytic enzyme. The enzymatic activity was optimal at a pH of 6.8 and at a temperature of 30 °C. Ethanol production from this recombinant enzyme was also analyzed on EglaRR01 crop residues, and resulted in conversion of cellulose from red rice into simple sugars which were further fermented by Saccharomyces cerevisiae to produce ethanol after seven days. Ethanol yield in this study was approximately 8 g/L. The gene found herein shows strong potential for use in ethanol production from cellulosic biomass (second generation ethanol). View Full-Text
Keywords: plant compost; functional metagenomics; endoglucanase plant compost; functional metagenomics; endoglucanase
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Meneses, C.; Silva, B.; Medeiros, B.; Serrato, R.; Johnston-Monje, D. A Metagenomic Advance for the Cloning and Characterization of a Cellulase from Red Rice Crop Residues. Molecules 2016, 21, 831.

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