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Molecules 2016, 21(6), 745; doi:10.3390/molecules21060745

Characterization of Secondary Metabolites from Purple Ipomoea batatas Leaves and Their Effects on Glucose Uptake

1
Department of Cosmeceutics, China Medical University, Taichung 40402, Taiwan
2
Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 40447, Taiwan
3
Department of Biological Science and Technology, China Medical University, Taichung 40402, Taiwan
4
Graduate Institute of Integrated Medicine, China Medical University, Taichung 40402, Taiwan
5
Proteomics Core Laboratory, Department of Medical Research, China Medical University Hospital, Taichung 40447, Taiwan
6
Department of Biochemistry, National Defense Medical Center, Taipei 11466, Taiwan
7
School of Pharmacy, China Medical University, Taichung 40402, Taiwan
8
Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 5 May 2016 / Revised: 28 May 2016 / Accepted: 3 June 2016 / Published: 8 June 2016
View Full-Text   |   Download PDF [3255 KB, uploaded 8 June 2016]   |  

Abstract

Ipomoea batatas has long been used in folk medicine for the treatment of hyperglycemia or as a food additive for the prevention of type 2 diabetes. However, neither the plant extract nor its active components have been evaluated systematically. In this work four crude extracts, including n-hexane- (IBH), 95% MeOH- (IBM), n-BuOH- (IBB), and H2O-soluble (IBW) fractions, were prepared by fractionation of a methanolic extract of purple I. batatas leaves. Twenty-four pure compounds 124 were then isolated by various chromatographic techniques and their structures identified from NMR and MS data. Glucose uptake assays in differentiated 3T3-L1 adipocytes and rat primary hepatocytes, as well as western blot analysis, were carried out to evaluate the antidiabetic activity of this species. The IBH crude fraction, with methyl decanoate (22) as a major and active compound, showed the greatest effect on glucose uptake, most likely via activation of Glut4 and regulation of the PI3K/AKT pathway. Quercetin 3-O-β-d-sophoroside (1), quercetin (3), benzyl β-d-glucoside (10), 4-hydroxy-3-methoxybenzaldehyde (12), and methyl decanoate (22) could be important components contributing to the antidiabetic effects. We conclude that purple I. batatas leaves have potential as an antidiabetic plant source and the active constituents 1, 3, 10, 12, and 22 are promising lead candidates for future investigation. View Full-Text
Keywords: Ipomoea batatas; purple sweet potato leaves; glucose uptake effect Ipomoea batatas; purple sweet potato leaves; glucose uptake effect
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Lee, C.-L.; Lee, S.-L.; Chen, C.-J.; Chen, H.-C.; Kao, M.-C.; Liu, C.-H.; Chen, J.-Y.; Lai, Y.-T.; Wu, Y.-C. Characterization of Secondary Metabolites from Purple Ipomoea batatas Leaves and Their Effects on Glucose Uptake. Molecules 2016, 21, 745.

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