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Molecules 2016, 21(6), 693; doi:10.3390/molecules21060693

Efficient Preparation of Streptochlorin from Marine Streptomyces sp. SYYLWHS-1-4 by Combination of Response Surface Methodology and High-Speed Counter-Current Chromatography

1
LiDakSum Marine Biopharmaceutical Research Center, Ningbo University, Ningbo 315211, China
2
Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China
3
Department of Biology, Northeastern University, Boston, MA 02115, USA
4
Ningbo Boao Bioengineering Corporation, Ningbo 315201, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 28 March 2016 / Revised: 16 May 2016 / Accepted: 23 May 2016 / Published: 27 May 2016
(This article belongs to the Section Natural Products)
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Abstract

Since first isolated from the lipophilic extract of Streptomyces sp. SF2583, streptochlorin, has attracted a lot of attention because of its various pharmacological properties, such as antibiotic, antiallergic, antitumor, and anti-inflammatory activities. For the efficient preparation of streptochlorin from a producing strain Streptomyces sp. SYYLWHS-1-4, we developed a combinative method by using response surface methodology (RSM) and high-speed counter-current chromatography (HSCCC). In the fermentation process, we used RSM to optimize the condition for the efficient accumulation of streptochlorin, and the optimal parameters were: yeast extract 1.889 g/L, soluble starch 8.636 g/L, K2HPO4 0.359 g/L, CaCl2 2.5 g/L, MgSO4 0.625 g/L, marine salt 25 g/L, medium volume 50%, initial pH value 7.0, temperature 27.5 °C, which enhanced streptochlorin yield by 17.7-fold. During the purification process, the preparative HSCCC separation was performed using a petroleum ether–ethyl acetate–methanol–water (9:0.8:5:5, v/v/v/v) biphasic solvent system, where 300 mg of crude sample yielded 16.5 mg streptochlorin with over 95% purity as determined by UPLC. Consequently, the combination method provided a feasible strategy for highly effective preparation of streptochlorin, which ensured the supply of large amounts of streptochlorin for in vivo pharmacological assessments or other requirements. View Full-Text
Keywords: streptochlorin; Streptomyces sp. SYYLWHS-1-4; response surface methodology; high-speed counter-current chromatography; preparation streptochlorin; Streptomyces sp. SYYLWHS-1-4; response surface methodology; high-speed counter-current chromatography; preparation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Li, L.; He, S.; Ding, L.; Yuan, Y.; Zhu, P.; Epstein, S.; Fan, J.; Wu, X.; Yan, X. Efficient Preparation of Streptochlorin from Marine Streptomyces sp. SYYLWHS-1-4 by Combination of Response Surface Methodology and High-Speed Counter-Current Chromatography. Molecules 2016, 21, 693.

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