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Molecules 2016, 21(6), 692; doi:10.3390/molecules21060692

Effects of Rhodomyrtus tomentosa Extract on Killing Activity of Human Neutrophils and Membrane Integrity of Enterohaemorrhagic Escherichia coli O157:H7

1
Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand
2
Excellent Research Laboratory on Natural products, Faculty of Science and Natural Product Research Center of Excellence, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand
*
Author to whom correspondence should be addressed.
Academic Editors: Anake Kijjoa, Bungorn Sripanidkulchai and Maria Emília de Sousa
Received: 20 February 2016 / Revised: 21 May 2016 / Accepted: 23 May 2016 / Published: 27 May 2016
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Abstract

Enterohaemorrhagic Escherichia coli (E. coli) O157:H7 is one of the most virulent causative agents of foodborne disease. Use of antibiotics for the treatment against E. coli O157:H7 infection leads to hemolytic uremic syndrome. The present study evaluated the potential of ethanolic leaf extract of a medicinal plant, Rhodomyrtus tomentosa in enhancing the killing activity of human neutrophils against E. coli O157:H7. In addition, the effects of the extract on membrane permeability of the organisms were studied. In the killing assay, percentage survival of the bacterial cells after being exposed to human neutrophils in the presence of various concentrations of the extract were determined. At 45 min, percentage survival of E. coli O157:H7 and E. coli ATCC 25922 after treated with neutrophils in the presence of the extract at 125–250 µg/mL was 58.48%–50.28% and 69.13%–35.35%, respectively. Furthermore, upon treatment with R. tomentosa at 250 µg/mL uptake of crystal violet by E. coli O157:H7 and E. coli ATCC 25922 was increased to 40.07% and 36.16%, respectively. Therefore, it is suggested that the extract exhibited dual effects as immunostimulant and membrane permeabilizing agent perhaps resulted in enhancing the killing activity of neutrophils against the organisms. View Full-Text
Keywords: Rhodomyrtus tomentosa; human neutrophils; Escherichia coli O157:H7; immunomodulatory activity; membrane integrity Rhodomyrtus tomentosa; human neutrophils; Escherichia coli O157:H7; immunomodulatory activity; membrane integrity
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MDPI and ACS Style

Hmoteh, J.; Syed Musthafa, K.; Pomwised, R.; Voravuthikunchai, S.P. Effects of Rhodomyrtus tomentosa Extract on Killing Activity of Human Neutrophils and Membrane Integrity of Enterohaemorrhagic Escherichia coli O157:H7. Molecules 2016, 21, 692.

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