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Molecules 2016, 21(5), 602; doi:10.3390/molecules21050602

Identification of New Epididymal Luminal Fluid Proteins Involved in Sperm Maturation in Infertile Rats Treated by Dutasteride Using iTRAQ

1
Department of Reproductive Pharmacology, Key Lab of Reproduction Regulation of NPFPC, SIPPR, IRD, Fudan University, Shanghai 200032, China
2
Department of Laboratory Medicine, Shanghai Eighth People’s Hospital, Shanghai 200233, China
3
Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai 200437, China
4
Ming Qi Natural Healthcare Center, New York, NY 10016, USA
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Wei-Zhu Zhong
Received: 15 March 2016 / Revised: 25 April 2016 / Accepted: 26 April 2016 / Published: 11 May 2016
(This article belongs to the Special Issue Drug Design and Discovery: Principles and Applications)
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Abstract

Background: Spermatozoa become mature and acquire fertilizing capacity during their passage through the epididymal lumen. In this study, we identified new epididymal luminal fluid proteins involved in sperm maturation in infertile rats by dutasteride, a dual 5α-reductase inhibitor, in order to provide potential epididymal targets for new contraceptives and infertility treatment. Methods: Male rats were treated with dutasteride for 28 consecutive days. We observed the protein expression profiles in the epididymal luminal fluids in infertile and normal rats using isobaric tags for relative and absolute quantitation (iTRAQ) technique. The confidence of proteome data was validated by enzyme-linked immunosorbent assays. Results: 1045 proteins were tested, and 23 of them presented different expression profiling in the infertile and normal rats. The seven proteins were down-regulated, and 16 proteins were up-regulated. Among the seven proteins which were significantly down-regulated by dutasteride in the epididymal luminal fluids, there were three β-defensins (Defb2, Defb18 and Defb39), which maybe the key proteins involved in epididymal sperm maturation and male fertility. Conclusions: We report for the first time that dutasteride influences the protein expression profiling in the epididymal luminal fluids of rats, and this result provides some new epididymal targets for male contraception and infertility therapy. View Full-Text
Keywords: dutasteride; rat epididymis; luminal fluids; iTRAQ; proteomics; drug targets; contraceptives; infertility therapy dutasteride; rat epididymis; luminal fluids; iTRAQ; proteomics; drug targets; contraceptives; infertility therapy
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Xie, S.-W.; Li, G.-T.; Qu, L.-J.; Cao, Y.; Wang, Q.; Zhou, J.-Y.; Zhong, R.-H.; Guo, X.-J.; Zhu, Y. Identification of New Epididymal Luminal Fluid Proteins Involved in Sperm Maturation in Infertile Rats Treated by Dutasteride Using iTRAQ. Molecules 2016, 21, 602.

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