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Molecules 2016, 21(5), 598; doi:10.3390/molecules21050598

The Role of fadD19 and echA19 in Sterol Side Chain Degradation by Mycobacterium smegmatis

1
Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Lodz, 12/16 Banacha Street, 90-237 Lodz, Poland
2
Institute of Medical Biology, Polish Academy of Sciences, 106 Lodowa Street, 93-232 Lodz, Poland
*
Authors to whom correspondence should be addressed.
Academic Editor: Carlo Siciliano
Received: 11 January 2016 / Revised: 20 April 2016 / Accepted: 2 May 2016 / Published: 6 May 2016
(This article belongs to the Section Molecular Diversity)
View Full-Text   |   Download PDF [1897 KB, uploaded 6 May 2016]   |  

Abstract

Mycobacteria are able to degrade natural sterols and use them as a source of carbon and energy. Several genes which play an important role in cholesterol ring degradation have been described in Mycobacterium smegmatis. However, there are limited data describing the molecular mechanism of the aliphatic side chain degradation by Mycobacterium spp. In this paper, we analyzed the role of the echA19 and fadD19 genes in the degradation process of the side chain of cholesterol and β-sitosterol. We demonstrated that the M. smegmatis fadD19 and echA19 genes are not essential for viability. FadD19 is required in the initial step of the biodegradation of C-24 branched sterol side chains in Mycobacterium smegmatis mc2155, but not those carrying a straight chain like cholesterol. Additionally, we have shown that echA19 is not essential in the degradation of either substrate. This is the first report, to our knowledge, on the molecular characterization of the genes playing an essential role in C-24 branched side chain sterol degradation in M. smegmatis mc2155. View Full-Text
Keywords: microbial sterol degradation; cholesterol; β-sitosterol; M. smegmatis; sterol side-chain degradation microbial sterol degradation; cholesterol; β-sitosterol; M. smegmatis; sterol side-chain degradation
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Wrońska, N.; Brzostek, A.; Szewczyk, R.; Soboń, A.; Dziadek, J.; Lisowska, K. The Role of fadD19 and echA19 in Sterol Side Chain Degradation by Mycobacterium smegmatis. Molecules 2016, 21, 598.

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