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Molecules 2016, 21(5), 569; doi:10.3390/molecules21050569

Using LC and Hierarchical Cluster Analysis as Tools to Distinguish Timbó Collections into Two Deguelia Species: A Contribution to Chemotaxonomy

Programa de Pós-Graduação em Química, Instituto de Ciências Exatas e Naturais, Universidade Federal do Pará, Campus Universitário do Guamá, 66075-970 Belém-PA, Brazil
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Academic Editor: Derek J. McPhee
Received: 22 March 2016 / Revised: 19 April 2016 / Accepted: 25 April 2016 / Published: 30 April 2016
(This article belongs to the Section Natural Products)
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Abstract

The species Deguelia utilis and Deguelia rufescens var. urucu, popularly known as “timbó,” have been used for many years as rotenone sources in insecticide formulations. In this work, a method was developed and validated using a high-performance liquid chromatography-photodiode array (HPLC-PDA) system, and results were analyzed using hierarchical cluster analysis (HCA). By quantifying the major rotenoids of these species, it was possible to establish a linear relation between them. The ratio between the concentrations of rotenone and deguelin for D. utilis is approximately 1:0.8, respectively, while for D. rufescens var. urucu it is 2:1. These results may help to distinguish these species contributing to their taxonomic identification. View Full-Text
Keywords: Deguelia; chemotaxonomy; rotenoids; high-performance liquid chromatography (HPLC); hierarchical cluster analysis (HCA) Deguelia; chemotaxonomy; rotenoids; high-performance liquid chromatography (HPLC); hierarchical cluster analysis (HCA)
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MDPI and ACS Style

da Costa, D.; e Silva, C.; Pinheiro, A.; Frommenwiler, D.; Arruda, M.; Guilhon, G.; Alves, C.; Arruda, A.; Da Silva, M. Using LC and Hierarchical Cluster Analysis as Tools to Distinguish Timbó Collections into Two Deguelia Species: A Contribution to Chemotaxonomy. Molecules 2016, 21, 569.

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