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Article

The Effects of Fungicide, Soil Fumigant, Bio-Organic Fertilizer and Their Combined Application on Chrysanthemum Fusarium Wilt Controlling, Soil Enzyme Activities and Microbial Properties

by
Shuang Zhao
1,†,
Xi Chen
1,†,
Shiping Deng
2,
Xuena Dong
1,
Aiping Song
1,
Jianjun Yao
3,
Weimin Fang
1 and
Fadi Chen
1,*
1
College of Horticulture, Nanjing Agricultural University, Nanjing 210000, China
2
Department of Plant and Soil Science, Oklahoma State University, Stillwater, OK 74078, USA
3
Shanghai Honghua Horticulture Co., Ltd., Shanghai 200000, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Molecules 2016, 21(4), 526; https://doi.org/10.3390/molecules21040526
Submission received: 25 December 2015 / Revised: 15 April 2016 / Accepted: 18 April 2016 / Published: 21 April 2016
(This article belongs to the Section Molecular Diversity)
Browse Figures
Versions Notes

Abstract

:
Sustained monoculture often leads to a decline in soil quality, in particular to the build-up of pathogen populations, a problem that is conventionally addressed by the use of either fungicide and/or soil fumigation. This practice is no longer considered to be either environmentally sustainable or safe. While the application of organic fertilizer is seen as a means of combating declining soil fertility, it has also been suggested as providing some control over certain soil-borne plant pathogens. Here, a greenhouse comparison was made of the Fusarium wilt control efficacy of various treatments given to a soil in which chrysanthemum had been produced continuously for many years. The treatments comprised the fungicide carbendazim (MBC), the soil fumigant dazomet (DAZ), the incorporation of a Paenibacillus polymyxa SQR21 (P. polymyxa SQR21, fungal antagonist) enhanced bio-organic fertilizer (BOF), and applications of BOF combined with either MBC or DAZ. Data suggest that all the treatments evaluated show good control over Fusarium wilt. The MBC and DAZ treatments were effective in suppressing the disease, but led to significant decrease in urease activity and no enhancement of catalase activity in the rhizosphere soils. BOF including treatments showed significant enhancement in soil enzyme activities and microbial communities compared to the MBC and DAZ, evidenced by differences in bacterial/fungi (B/F) ratios, Shannon–Wiener indexes and urease, catalase and sucrase activities in the rhizosphere soil of chrysanthemum. Of all the treatments evaluated, DAZ/BOF application not only greatly suppressed Fusarium wilt and enhanced soil enzyme activities and microbial communities but also promoted the quality of chrysanthemum obviously. Our findings suggest that combined BOF with DAZ could more effectively control Fusarium wilt disease of chrysanthemum.

1. Introduction

Chrysanthemum (Chrysanthemum morifolium) is a commercially valuable ornamental species worldwide. Increasing urbanization and an improving standard of living is stimulating the demand for both cut flowers and pot plants in China, prompting a rise in the monoculture-based production of chrysanthemum. As for most crop species, long-term continuous monoculture-based production results in serious continuous cropping obstacles of the chrysanthemum [1,2]. In the monoculture-based production system, chrysanthemum is generally affected by the Fusarium wilt, caused by Fusarium oxysporum f. sp. chrysanthemi (F. oxysporum), which is considered to be the most important soil-borne facultative pathogen, causing economically important losses of chrysanthemum and limiting chrysanthemum production [3].
The two most effective current interventions are the fungicide carbendazim (MBC) and the soil fumigant dazomet (DAZ), but neither of these compounds is considered to be environmentally friendly and their sustained use may have negative effects on soil ecosystem [4,5]. Microbial communities and diversity in soil are seen to be critical for the maintenance of soil health and quality, while soil enzyme activities are often used as indices of microbial growth and activity in soils [6,7,8]. Soil microbial properties and enzyme activities are closely related because transformations of the important organic elements occur through microorganisms [9,10]. In addition, the repeated application of fungicides inevitably suppresses the population of beneficial soil microbes and encourages the development of genetic resistance in the pathogen. As a result, efforts are being made to elaborate biological control methods, one of which involves bio-organic fertilizers (BOFs) [11,12,13,14].
The use of BOFs has been demonstrated to reduce the incidence of soil-borne disease affecting a range of plants [12,15], although how it achieves this is unclear. Here, the effectiveness of BOFs, particularly in combination with fungicide or soil fumigation, to control chrysanthemum Fusarium wilt has been explored. The demonstration that this treatment regime can suppress the disease should guide the elaboration of strategies enabling the sustainable and commercially viable production of chrysanthemum, while at the same time conserving the health of the soil ecosystem.

2. Results

2.1. The Impact of Fusarium Wilt on Chrysanthemum Growth

Each of the treatments significantly reduced the incidence of Fusarium wilt (Figure 1a) and increased the disease reduction percentage (DRP) (Figure 1b). After the 90 days growth of the third cropping period (February 2012), the DI was 19.8% in the CK plots, 9.4% in the BOF plots and <4% in the other plots. The DRP was 0% in the CK plots, 89% in the MBC plots, 84% in the DAZ plots, 81% in the MBC + BOF plots, 84% in the DAZ + BOF plots, and 53% in the BOF plots (Figure 1b). Shoot height were greatest for plants exposed to the DAZ + BOF treatment, followed by MBC + BOF and BOF, and lowest in the CK treatment (Table 1). It was significantly greater in the DAZ + BOF treatment than in either the DAZ or the BOF ones. MBC was not significantly different from that recorded in the DAZ treatment. A similar response was recorded for all the shoot, leaf and flower traits, excluding the number of ray floret.

2.2 Enzymatic Activity

Soil catalase activity (Figure 2a) was significantly altered in the BOF including treatments when compared to the CK treatment, while those of both urease (Figure 2b) and sucrase (Figure 2c) were significantly affected by each of the treatments. Catalase activity was highest in the DAZ + BOF treatment (7.2 fold that of the CK soil), followed by the BOF treatment (6.1 fold) and the MBC + BOF treatment (4.6 fold). The behavior of urease was quite different: the MBC and DAZ treated soils displayed a reduced activity (respectively, 65% and 61% of the CK soil level), while the activity level was doubled in the BOF treated soil. The urease activity was also enhanced in the MBC + BOF and DAZ + BOF treated soils. Sucrase activity was highest in the BOF treated soil and lowest in the MBC treated soil if excluding CK. When BOF was combined with either MBC or DAZ, sucrase activity was suppressed compared to the level displayed in the BOF treatment.

2.3. Diversity of the Soil Microbiota

Based on the real-time PCR outputs, all of the treatments led to an increased presence of bacteria but a decreased presence of fungi, resulting in a marked increase in the ratio of bacteria to fungi of up to 30.5 (Table 2). The DAZ + BOF treatment was the most conducive for bacterial growth, followed by MBC+BOF and BOF, while the DAZ treatment was the most suppressive for fungi. The bacterial and fungal DGGE profiles fell into six and seven clusters, respectively (Figure 3). The bacterial (Figure 3a) communities of BOF were clustered together at the level of the similarity coefficient of 61%, while the fungal communities (Figure 3b) in those soils in which DAZ + BOF formed part of the treatment were clustered together with the similarity coefficient of 52%, except #13 (40%), this may have been due to an acceptable soil sampling variation. The number of fragments detected ranged from 24 to 35 (bacteria) and 5 to 24 (fungi) (Table 3). H′ values suggested rather limited variation in the bacterial community (a range in H′ from 3.12 to 3.33), while the range relating to the fungal component was 1.44~3.00. The highest bacterial H′ value was recovered in the soils treated with BOF, whereas the highest fungal one was present in the non-treated control soil. The J metric was in general higher for the bacterial than for the fungal component. All of the treatments showed no significant differences on J related to the bacterial evenness, but there was no such consistency for the fungal community (Table 3).
According to the NCBI taxonomy database, 30 bacterial DGGE fragments were excised for sequencing, only one shared complete homology with a known sequence, present in a Flavobacterium sp. (Table 4). The remaining 29 were 94%–99% homologous with known sequences; 14 involved sequences present in uncultured bacteria, and the other 15 to sequences present in Alpha proteobacterium, Rhizobium sp., Variovorax sp., Anoxybacillus, Microbacterium sp., Sphingobium yanoikuyae, Flavobacterium sp., Dechlorospirillum sp., Ochrobactrum sp. and Clostridium neonatale. Of the 21 sequenced fungal DGGE fragments, one was fully homologous with an Ophiobolus herpotrichus sequence (Table 5), while 18 of the remaining 20 shared 96%–99% homology with sequences present in Aspergillus terreus, Leptosphaeria maculans, Fusarium sp., O. herpotrichus, Phoma sp. and Phoma macrostoma; the sequences of the other two shared homology with sequences present in uncultured soil fungi.

3. Discussion

The control of Fusarium wilt is a priority for the sustainable production of chrysanthemum. Utilization of compost to reduce the addition of chemical fertilizers and fungicides in plant protection is a promising strategy for both the present and future. The inclusion of BOF among the soil treatments evaluated was based on the expectation that it could not just shift the composition of the soil microbiota in a way which suppresses the Fusarium pathogen, but also could enhance soil quality and thereby plant productivity. In the event, the treatment that combined BOF with a moderate application of DAZ was the most effective in controlling the disease, improving the growth of the chrysanthemum plants, and maintaining the diversity of the soil microbiota.

3.1. The Effects of the Soil Treatments on Fusarium Wilt Incidence

The combined BOF/MBC and BOF/DAZ treatments’ superiority are fully in line with similar reports showing that combining chemicals with a biocontrol agent can effectively suppress soil pathogens [4,15]. One suggestion is that the provision of BOF supports the growth of pathogen antagonists [16], however the treatment involving BOF on its own did not markedly reduce the incidence of Fusarium wilt. This failure may have been due to the presence of a high pathogen load in the soil, given that chrysanthemum had been continuously cropped at the site for many years, so that any beneficial effect of the BOF on the growth of the pathogen antagonists may have been insufficient to overcome the pathogen pressure [16,17].
The applications of either MBC or DAZ on their own lowered the disease incidence and heighten the disease reduction percentage, but both interventions were significantly inhibitory over the urease enzyme activity of the soil microbiota. Because many soil enzymes are highly responsive to soil disturbance, their activity has been used as an index of environmental stability and soil quality. Given that the urease activity tended to fall in response to either MBC or DAZ, it is important that temporal changes in enzyme activity should been considered when assessing the sustainability of a disease control intervention.
In contrast, the application of BOF enhanced catalase, sucrase and urease activity. Previous studies have shown that both fungicide application and soil fumigation tend to reduce soil enzyme activity, presumably because of the treatments’ downward pressure on the viability of soil microbiota [7,18,19,20].

3.2. The Effect of the Various Soil Treatments on Soil Microbiota Composition

The DGGE-based diversity profiling indicated that BOF and various combinations of fungicides significantly affected the structure of the soil microbiota. The reduced number of fungal DGGE fragments detected in the BOF/DAZ and BOF/MBC treated soil showed that species richness was compromised, but not as severely as in soil treated with MBC. The same conclusions could be drawn from the behavior of the other diversity metrics H′ and J, supporting the observation reported by [21] that BOF application encourages the growth of Paenibacillus spp., but decreases the level of fungi diversity. The likelihood is that the organic nutrients supplied by the manure favored the growth of particular soil microbiota species [21,22].
The application of BOF induced significant changes to the composition of the soil microbiota. Most of the 30 sequenced bands of 16S rDNA in this study were related to soil bacterial such as S. yanoikuyae, Mycobacterium gilvum, Variovorax sp. and C. neonatale, along with species in the genera Dechlorospirillum, Ochrobactrum and Flavobacterium. According to the literature, both Microbacterium sp. and Sphingobium are effective degraders of certain herbicides [23,24]. S. yanoikuyae is an efficient carbazole-degrading organism which has been exploited in the context of a number of biotechnology applications [25,26]. A strain of Variovorax sp. has been shown to be capable of using certain fungicides as a source of energy and nutrients [27]. On the basis of the fungal-specific 18 S rRNA assay, most of the 21 sequenced bands of BOF including treatments were related to soil fungi such as L. maculans, O. herpotrichus, Aspergillus ochraceus, P. macrostoma and various Fusarium sp. and other Phoma sp. These fungi are common in soil and many of them have been associated with phytopathogenesis [28,29].
The combined BOF/DAZ treatment resulted in a large increase to the B/F ratio, which reached 1.6 fold that resulting from the DAZ treatment and 1.5 fold that resulting from the BOF/MBC treatment (Table 2); possible explanations for this are that the combined BOF/DAZ treatment changed the composition of the soil microbiota [13,16,30]. BOF has been reported to have significant effects on population of pathogenetic fungi in cucumber [31,32], banana [14,33], cotton [13], and watermelon [16]. In watermelon, for example, the provision of BOF during the seedling stage can significantly attenuate the density of soil pathogens, an observation that suggests the possibility that antibiotics are being produced by antagonistic microorganism [28,29]. In cotton, BOF effectively controls Verticillium wilt in cotton by suppressing the size of the V. dahlia population [30]. In addition, fungicides act by inhibiting fungal growth [4,34], with consequential effects on the B/F ratio.

3.3. The Effect of the Various Treatments on Chrysanthemum Productivity

The major finding from the present experiments has been that combining BOF with a modest dose of DAZ or MBC encouraged plant growth more strongly than was achievable via the use of MBC or DAZ on its own. The combined treatment significantly enhanced both vegetative and reproductive growth, as has been similarly shown in watermelon plants challenged by Fusarium oxysporum [16]. The effect is likely related to a combination of the nutrients present in the BOF, the activity of pathogen antagonists that thrive in the BOF, and the production of antibiotics produced by members of the Paenibacillus genus [21,28,29]. Organic amendments are routinely used to improve soil structure and plant nutrition; the BOF used provided a ready source of nutrients to support both plant growth and the establishment of Paenibacillus spp. populations. Certain peptides produced by P. polymyxa (SQR-21) have demonstrated antibiosis against F. oxysporum and other pathogenic fungi [28].

4. Materials and Methods

4.1. Site Description and Plant Material

The experimental site was located in Shanghai, China. Prior to the initiation of the trial, chrysanthemum production had been carried out uninterrupted over nine years without any chemical intervention against soil-borne disease, and the incidence of Fusarium wilt was high. From April 2011 to February 2012, three consecutive croppings (April to June 2011, August to October 2011, and December 2011 to February 2012) of chrysanthemum were grown. The soil (a sandy loam) had a pH of 7.2, and an average content of 11.3 g organic matter·kg−1, 1.32 g·N·kg−1, 0.11 g available P·kg−1, and 8.18 g available K·kg−1. Seedlings of the chrysanthemum cultivar ‘Jinba’ (provided by Honghua Horticulture Co. Ltd., Shanghai, China) were established in a greenhouse in a perlite medium at spacing of 20 cm for three weeks under a 16 h photoperiod and a relative humidity of 70%, with the daytime temperature held at 28 °C and the nighttime temperature at 22 °C.

4.2. BOF and Fungicide and Soil Fumigant

Prior to its incorporation in the soil, the antagonistic microbe Paenibacillus polymyxa SQR21 (a bacterial strain known to be a highly efficient antagonist against F. oxysporum) was used to prepare bioorganic fertilizer (BOF) [17]. The antagonist was grown in beef extract and peptone liquid culture on a shaker at 170 rpm at 30 °C for 2–3 days. This culture was then used directly to prepare the BOF product as described below. Organic fertilizer (OF), used for the BOF product, was composed of amino acid fertilizer and pig manure compost (1:1, w/w). Amino acid fertilizer was made from oil rapeseed cakes that were enzymatically hydrolyzed by aerobic microbial fermentation at <50 °C for 7 days. This amino acid fertilizer was containing 44.2% organic matter and 12.9% of amino acids, small molecular peptides and oligo peptides. The nutrient contents were 4.4% nitrogen (N), 5.27% available P, and 0.78% available K. Pig manure compost was made by Tian-niang Ltd. (Yixing, China) in Suzhou by composting pig manure at a temperature range of 30–70 °C for 25 days. This compost was contained of 30.4% organic matter, 2.0% N, 1.6% available P, and 1.2% available K. The organic fertilizer, which was enriched with P. polymyxa SQR21, was named bio-organic fertilizer (BOF).
The BOF used in this experiment was obtained by aerobically fermenting OF with the SQR21 for 6 days at <45 °C. The mature BOF product contained approximately 5 × 109 CFU·g−1 dry weight (DW) of SQR21, the nutrient contents were 59% organic matter, 5.1% N, 5.8% available P, and 1.23% available K. The BOF product was stored at less than 25 °C prior to use in experiments [17].
The fungicide carbendazim (methyl 2-benzimidazole carbamate, MBC, pure ≥ 99.5%) 50% WP and the soil fumigant dazomet (3,5-dimethyl-1,3,5-thiadiazinane-2-thione, DAZ, pure ≥ 95.0%) were purchased from Yuelian Chemical Industry Co. Ltd. (Shanghai, China) and Shizhuang Chemical Industry Co. Ltd. (Nantong, China).

4.3. Soil Treatment and Sampling

The experiment was a randomized complete block design with three replicates and six treatments, which resulted in a total of 18 plots. Before planting, the soil was plowed to a 25 cm depth. Each plot was 80 cm × 100 cm, holding 80 plants. The six treatments were: (1) an untreated control (CK); (2) 25 g MBC per m2 applied by watering the soil; (3) 30 g DAZ per m2 soil, applied by mixing the DAZ microgranules into the top 15 cm of the soil, after which the soil was covered by a plastic membrane for 20 days and left exposed for a further 7 days prior to planting; (4) 25 g MBC per m2 + 2.5 kg BOF per m2, 7 days after treated soil with 25 g MBC per m2, soil was further amended with the BOF at a rate of 2.5 kg·m−2; (5) 30 g DAZ + 2.5 kg BOF per m2, after treated soil with 30 g DAZ per m−2 (DAZ application method as described in treatment 3), soil was further amended with the BOF at a rate of 2.5 kg·m−2; and (6) 2.5 kg BOF per m2. Finally, chrysanthemums from the third croppings with various treatments were obtained in February 2012. Ten plants were sampled randomly from each replicate 90 days after transplanting (February 2012). The adhering soil was gently shaken off each sampled plant, passed through a 2 mm sieve and stored at 4 °C prior to analysis.

4.3.1. The Measurement of Growth and Disease Incidence

Plant growth was monitored with respect to shoot height and diameter, shoot fresh and dry weight, leaf width and length, flower diameter and ray floret number. Leaf chlorophyll content (SPAD value) was quantified using a SPAD-502 Plus chlorophyll meter (Top Instrument Co., Hangzhou, China). Infection by F. oxporum was checked on a daily basis. A disease incidence (DI) score for each plot was assigned as the ratio of infected plants present at 90 days (January 2012), and the disease reduction percentage (DRP) was given by the expression (1 − DT/DC) × 100, where DC and DT were the DI values in the CK and treatment plots, respectively (Ling et al., 2012).

4.3.2. Soil DNA Extraction and PCR Amplification

DNA was extracted from the soil samples in triplicate, using an Ultra Clean™ Soil kit (MOBIO Laboratories, Carlsbad, CA, USA). The DNAs from the replicates were pooled and subjected to a spectrophotometric evaluation of quality and concentration using a NanoVue device (GE Life Sciences, Piscataway, NJ, USA). To detect the fungal content of the soil DNA, the ITS region of the fungal 18S rRNA sequence was amplified from this DNA using the primer pair ITS1-F/ITS2 [17,35]. Each 50 µL PCR contained 5 µL 10× PCR buffer (Mg2+ free), 3 µL 25 mM MgCl2, 4 µL 2.5 mM dNTP, 1 U Taq DNA polymerase (Takara, Dalian, China), 2 µL of each primer (10 nM) and 4 µL DNA template (25 ng/µL). The PCR regime comprised a 95 °C/5 min denaturation, followed by 35 cycles of 95 °C/50 s, 57 °C/60 s, 72 °C/60 s, and was completed by final extension of 72 °C/10 min. Similar reactions were used to amplify a fragment of the bacterial 16S rRNA sequence, using the primer pair Eub338/Eub518 [36]. A GC clamp (5′-CGCCCGCCGCGCGCGG CGGGCGGGG CGGGGGACGGGGGG) was added to the 5′ end of both ITS1-F and Eub338 to stabilize the melting behavior of the amplicon. The PCR products were purified using an Axyprep™ DNA gel extraction kit (Axygen Biotechnology Limited, Hangzhou, China) prior to their electrophoretic separation.

4.3.3. Denaturing Gradient Gel Electrophoresis (DGGE) Profiling

The 18S and 16S rRNA amplicons were subjected to DGGE using the D-Code DGGE system (Bio-Rad, CA, USA). A 20 µL aliquot of the PCR product (containing ~400 ng DNA) was loaded onto each lane of a DGGE gel; for the fungal amplicons, the polyacrylamide concentration was 8% (37.5:1 acrylamide/bisacrylamide) and the denaturing gradient was 25%~45%; for the bacterial amplicons, the polyacrylamide concentration was 7% (37.5:1 acrylamide/bisacrylamide) and the denaturing gradient was 30%–70%. The electrolyte was 40 mM Tris acetate, 1 mM EDTA, pH 8.0. The temperature was held at 60 °C and the voltage at 80 C for 16 h. The amplicons were visualized by silver staining, following [17]. Prominent fragments were excised and sequenced.

4.3.4. Real-Time PCR

Real-time PCR was conducted to estimate the abundance of bacterial and fungal species. The reactions were based on 2× TaKaRa SYBR® Premix Taq™ (Bio technology Dalian Co. Ltd., Dalian, China) using a Mastercycler ep realplex 2S device (Eppendorf, Germany). The primer pairs were Eub338/Eub518 (bacteria) and ITS1-F/ITS2 (fungi). Each 25 μL reaction contained 10 µL 2× SYBR® Premix ExTaq™, 0.5 µL of each primer, 0.5 μL 50× ROX Reference Dye II and 2 µL DNA. For the bacterial assay, the PCR comprised an initial denaturation of 95 °C/10 min, followed by 40 cycles of 95 °C/10 s, 54 °C/10 s, 72 °C/30 s, while for the fungal amplicons, the initial denaturation step was 95 °C/5 min and the cycling regime was 45 cycles of 95 °C/15 s, 53 °C/30 s, 72 °C/45 s. Standard curves were generated using a 10-fold serial dilution (from 109 to 104 copies) of a plasmid containing a full length copy of Saccharomyces cerevisiae 18S rRNA or Escherichia coli 16S rRNA [3,14]. Plasmid was extracted by Axyprep™ Plasmid Miniprep Kit (Axygen, Hangzhou, China). Each biological sample was subjected to three technical replicates.

4.3.5. Enzymatic Activity

Catalase activity was determined using a titrimetric method [37]. The residual H2O2 was determined by titrated with KMnO4 in the presence of H2SO4 after 30 min of reaction. Catalase activity was expressed as mL 0.1 mol·L−1 KMnO4 consumed g−1·soil day−1. Urease activities were determined according to a method described by Liu et al. [38] and Fang et al. [39]. Urease activity was expressed as mg NH3–N released g−1·soil day−1. Sucrase activity was determined by a method described by Guan [37] with minor modifications. Briefly, a 5 g soil sample was treated by the addition 1 mL of toluene, followed by 15 mL sucrose and 5 mL phosphate buffer [mix 94.6 mL of Solution A (dissolve 13.61 g of KH2PO4 in water and dilute to 1 L) and 3.6 mL of Solution B (dissolve 35.81 g of NaH2PO4 in water and dilute to 1 L, pH 5.5)]. After incubation at 37 °C for 24 h, the suspension was filtered through Whatman 1001-090 filter paper (Whatman International, Maidstone, UK) and 0.5 mL of filtrate was treated with 1.5 mL salicylic acid and held for 5 min at 100 °C. Once the solution had cooled, sufficient deionized water was added to make the volume up to 25 mL. The intensity of the pink color that developed after 60 min was measured spectrophotometrically at 508 nm, using a UV2300 device (Shanghai Zhongchen instrument Co., Shanghai, China). The quantity of reducing sugar released by sucrase activity was determined by reference to a calibration curve and expressed as mg glucose g−1·soil day−1.

4.4. Statistical Analyses

One- and two-way analyses of variance (ANOVA) were conducted to compare enzymatic activities and microbial abundance in the soil samples. The Duncan multiple range test was used to assign significance to differences (p < 0.05) between treatment means. All statistical analyses were carried out using SPSS v20.0 software (SPSS, Chicago, IL, USA). Silver stained DGGE bands were analyzed with Quantity One computer soft-ware (version 4.6.3, Bio-Rad, Hercules, CA, USA). The relative intensity of individual DGGE fragments was expressed as the ratio between the intensity of that band and the total intensity of all bands in that lane. A Shannon index (H′) was calculated from the DGGE profiles following [40]. The Shannon–Weaver equitability index (J) was derived from the expression H′/(Ln ni), where ni was number of species detected in a given soil sample.

5. Conclusions

Treatment with either fungicide or soil fumigation, and particularly in combination with the provision of BOF, provided good control over Fusarium wilt. BOF including treatments show good performance in producing the best quality of chrysanthemum, but the BOF/DAZ combination was the most efficacious in terms of increasing the B/F ratio and enriching the diversity of the soil microbiota. These results will guide the improvement of strategies against Fusarium wilt of chrysanthemum.

Acknowledgments

This work was, in part, supported by Fund for Independent Innovation of Agriculture Sciences in Jiangsu Province [CX(12)2020], National Natural Science Foundation of China for Youth (31301809), Foundation for Six Talents Peak of Jiangsu Province (2013-NY-022), National Basic Research Program of China (KYZ201419 and KYCYL201501) and Postdoctoral Science Foundation of China (2014M551613) .

Author Contributions

S.Z., F.C. and W.F. conceived and designed the experiments; X.C. performed the experiments; X.D. and A.S. analyzed the data; J.Y. contributed reagents/materials/analysis tools; S.Z. and S.D. wrote the paper.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Duineveld, B.M.; Rosado, A.S.; van Elsas, J.D.; van Veen, J.A. Analysis of the dynamics of bacterial communitiesin the rhizosphere of chrysanthemum via denaturing gradient gelelectrophoresis and substrate utilization patterns. Appl. Environ. Microbiol. 1998, 64, 4950–4957. [Google Scholar] [PubMed]
  2. Duineveld, B.M.; Kowalchuk, G.A.; Keijzer, A.; van Elsas, J.D.; van Veen, J.A. Analysis of bacterial communities in the rhizosphere of chrysanthemum via denaturing gradient gel electrophoresis of PCR-Amplified 16S rRNA as well as DNA fragments coding for 16S rRNA. Appl. Environ. Microbiol. 2001, 67, 172–178. [Google Scholar] [CrossRef] [PubMed]
  3. Farias, M.F.; Saad, J.C.C. Growth and quality of chrysanthemum produced in greenhouse. Hortic. Bras. 2005, 23, 740–742. [Google Scholar] [CrossRef]
  4. Niewiadomska, A.; Sawicka, A. Effect of carbendazim, imazetapir and thiram on nitrogenase activity, number of microorganisms in soil and yield of hybrid lucerne (Medicago media). Pol. J. Environ. Stud. 2002, 11, 737–744. [Google Scholar]
  5. Nannipieri, P.; Grego, S.; Ceccanti, B. Ecological significance of the biological activityin soil. In Soil Biochemistry; Bollag, J.M., Stotzky, G., Eds.; Marcel Dekker: New York, NY, USA, 1990; Volume 6, pp. 293–355. [Google Scholar]
  6. Chen, S.K.; Edwards, C.A.; Subler, S. Effect of fungicides benomyl, captan and chlorothalonil on soil microbial activity and nitrogen dynamics in laboratory incubations. Soil Biol. Biochem. 2001, 33, 1971–1980. [Google Scholar] [CrossRef]
  7. Nannipieri, P.; Giagnoni, L.; Lagomarsino, G.; Puglisi, E.; Ceccanti, B.; Masciandaro, G.; Fornasierm, F.; Moscatelli, M.C.; Marinari, S. Soil enzymology: classical and molecular approaches. Biol. Fertil. Soils 2012, 48, 743–762. [Google Scholar] [CrossRef]
  8. Ai, Liang; Sun, C.G.J.; Wang, X.; Zhou, W. Responses of extracellular enzyme activities and microbial community in both the rhizosphere and bulk soil to long-term fertilization practices in a fluvo-aquic soil. Geoderma 2012, 330–338, 173–174. [Google Scholar]
  9. Speir, T.W.; Lee, R.; Pansier, E.A.; Cairns, A. A comparison of sulphatase, urease and protease activities in planted and in fallow soils. Soil Biol. Biochem. 1980, 12, 281–291. [Google Scholar] [CrossRef]
  10. Frankenberger, W.T.; Dick, W.A. Relationships between enzyme ctivities and microbial growth and activity in soil. Soil Sci. Soc. Am. J. 1983, 47, 945–951. [Google Scholar] [CrossRef]
  11. Zhang, S.; Raza, W.; Yang, X.M.; Hu, J.; Huang, Q.W.; Xu, Y.C.; Liu, X.; Ran, W.; Shen, Q.R. Control of Fusarium wilt disease of cucumber plants with the application of a bioorganic fertilizer. Biol. Fertil. Soils 2008, 4, 1073–1080. [Google Scholar] [CrossRef]
  12. Zhang, N.; Wu, K.; He, X.; Li, S.Q.; Zhang, Z.H.; Shen, B.; Yang, X.M.; Zhang, R.F.; Huang, Q.W.; Shen, Q.R. A new bioorganic fertilizer can effectively control banana wilt by strong colonization of Bacillus subtilis N11. Plant Soil 2011, 344, 87–97. [Google Scholar] [CrossRef]
  13. Luo, J.; Ran, W.; Hu, J.; Yang, X.M.; Xu, Y.C.; Shen, Q.R. Application of bio-organic fertilizer significantly affected fungal diversity of soils. Soil Sci. Soc. Am. J. 2010, 74, 2039–2048. [Google Scholar] [CrossRef]
  14. Shen, Z.Z.; Zhong, S.T.; Wang, Y.G.; Mei, X.L.; Wang, B.B.; Li, R.; Ruan, Y.Z.; Shen, Q.R. Induced soil microbial suppression of banana Fusarium wilt disease using compost and bio fertilizers to improve yield and quality. Eur. J. Soil Biol. 2013, l57, 1–8. [Google Scholar] [CrossRef]
  15. Minuto, A.; Davide, S.; Garibaldi, A.; Gullino, M.L. Control of soil-borne pathogens of tomato using a commercial formulation of Streptomyces griseoviridis and solarization. Crop Prot. 2006, 25, 468–475. [Google Scholar] [CrossRef]
  16. Ling, N.; Zhang, W.W.; Tan, S.Y.; Huang, Q.W.; Shen, Q.R. Effect of the nursery application of bioorganic fertilizer on spatial distribution of Fusarium oxysporum f. sp. niveum and its antagonistic bacterium in the rhizosphere of watermelon. Appl. Soil Ecol. 2012, 59, 13–19. [Google Scholar] [CrossRef]
  17. Zhao, S.; Liu, D.Y.; Ling, N.; Chen, F.; Fan, W.; Shen, Q.R. Bio-organic fertilizer application significantly reduces the Fusarium oxysporum population and alters the composition of fungi communities of watermelon Fusarium wilt rhizosphere soil. Biol. Fertil. Soils 2014, 50, 76. [Google Scholar] [CrossRef]
  18. Dick, R.P. Soil enzyme activities as indicators of soil quality. In Defining Soil Quality for A Sustainable Environment; No. 35.; Doran, J.W., Coleman, D.C., Bezdicek, D.F., Stewart, B.A., Eds.; SSSA Special Publication: ASA, CSSA, SSA, Madison, WI, USA, 1994; pp. 107–124. [Google Scholar]
  19. Bending, G.D.; Turner, M.K.; Rayns, F.; Marx, M.C.; Wood, M. Microbial and biochemical soil quality indicators and their potential for differentiating areas under contrasting agricultural management regimes. Soil Biol. Biochem. 2004, 36, 1785–1792. [Google Scholar] [CrossRef]
  20. Ingram, C.W.; Coyne, M.S.; Williams, D.W. Effects of commercial diazinon and imidacloprid on microbial urease activity in soil. J. Environ. Qual. 2005, 34, 1573–1580. [Google Scholar] [CrossRef] [PubMed]
  21. Ling, N.; Wang, D.S.; Zhu, C.; Song, Y.; Yu, G.H.; Ran, W.; Huang, Q.W.; Guo, S.W.; Shen, Q.R. Response of the population size and community structure of Paenibacillus spp. to different fertilization regimes in a long-term experiment of red soil. Plant Soil 2014, 383, 87–98. [Google Scholar] [CrossRef]
  22. Ling, N.; Sun, Y.M.; Ma, J.H.; Guo, J.J.; Zhu, P.; Peng, C.; Yu, G.H.; Ran, W.; Guo, S.W.; Shen, Q.R. Response of the bacterial diversity and soil enzyme activity in particle-size fractions of Mollisol after different fertilization in along-term experiment. Biol. Fertil. Soils. 2013, 50, 901–911. [Google Scholar] [CrossRef]
  23. Boldrin, B.; Tiehm, A.; Fritzsche, C. Degradation of phenanthrene, fluorene, fluoranthene, and pyrene by a Mycobacterium sp. Appl. Environ. Microbiol. 1993, 59, 1927–1930. [Google Scholar] [PubMed]
  24. Gai, Z.H.; Gai, Z.; Yu, B.; Li, L.; Wang, Y.; Ma, C.; Feng, J.; Deng, Z.; Xu, P. Cometabolic degradation of dibenzofuran and dibenzothiophene by a newly isolated carbazole-degrading Sphingomonas sp. strain. Appl. Environ. Microbiol. 2007, 73, 2832–2838. [Google Scholar] [CrossRef] [PubMed]
  25. Dussault, M.; Becaert, V.; Francois, M.; Sauve, S.; Deschenes, L. Effect of copper on soil functional stability measured by relative soil stability index (RSSI) based on two enzyme activities. Chemosphere 2008, 72, 755–762. [Google Scholar] [CrossRef] [PubMed]
  26. Cunliffe, M.; Kertesz, M.A. Effect of Sphingobium yanoikuyae B1 inoculation on bacterial community dynamics and polycyclic aromatic hydrocarbon degradation in aged and freshly PAH-contaminated soils. Environ. Pollut. 2006, 144, 228–237. [Google Scholar] [CrossRef] [PubMed]
  27. Sørensen, S.R.; Albers, C.N.; Aamand, J. Rapid mineralization of the phenylurea herbicide diuron by Variovorax sp. strain SRS16 in pure culture and within a two-member consortium. Appl. Environ. Microbiol. 2008, 74, 2332–2340. [Google Scholar] [CrossRef] [PubMed]
  28. Dijksterhuis, J.; Sanders, M.; Gorris, L.G.M.; Smid, E.J. Antibiosis plays a role in the context of direct interaction during antagonism of Paenibacillus polymyxa towards Fusarium oxysporum. J. Appl. Microbiol. 1999, 86, 13–21. [Google Scholar] [CrossRef] [PubMed]
  29. Raza, W.; Wang, Y.; Shen, Q. R. Paenibacillus polymyxa: antibiotics, hydrolytic enzymes and hazard assessment. J. Plant Pathol. 2008, 90, 419–430. [Google Scholar]
  30. Lang, J.J.; Hu, J.; Ran, W.; Xu, Y.C.; Shen, Q. Control of cotton Verticillium wilt and fungal diversity of rhizosphere soils by bio-organic fertilizer. Biol. Fertil. Soils 2012, 48, 191–203. [Google Scholar] [CrossRef]
  31. Chen, L.H.; Huang, X.Q.; Zhang, F.G.; Zhao, D.K.; Yang, X.M.; Shen, Q.R. Applications of Trichoderma harzianum SQR-T037 bioorganic fertilizer significantly affect the microbial communities of continuously cropped soil of cucumber. J. Sci. Food Agric. 2012, 92, 2465–2470. [Google Scholar] [CrossRef] [PubMed]
  32. Qiu, M.H.; Zhang, R.F.; Xue, C.; Zhang, S.S.; Li, S.Q.; Zhang, N.; Shen, Q.R. Application of a novel bioorganic fertilizer can control Fusarium wilt by regulating microbial community of cucumber rhizosphere soils. Biol. Fertil. Soils 2012, 48, 807–816. [Google Scholar] [CrossRef]
  33. Wang, B.B.; Yuan, J.; Zhang, J.; Shen, Z.Z.; Zhang, M.X.; Li, R.; Ruan, Y.Z.; Shen, Q.R. Effects of novel bioorganic fertilizer produced by Bacillus amyloliquefaciens W19 on antagonism of Fusarium wilt of banana. Biol. Fertil. Soils 2013, 49, 435–446. [Google Scholar] [CrossRef]
  34. Burrows, L.A.; Edwards, C.A. The use of integrated soil microcosms to assess the impact of carbendazim on soil ecosystems. Ecotoxicology 2004, 13, 143–161. [Google Scholar] [CrossRef] [PubMed]
  35. Gardes, M.; Bruns, T.D. ITS primers with enhanced specificity for basidiomycetes, application to the identification of mycorrhizae and rusts. Mol. Ecol. 1993, 2, 113–118. [Google Scholar] [CrossRef] [PubMed]
  36. Fierer, N.; Jackson, J.A.; Vilgalys, R.; Jackson, R.B. Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays. Appl. Environ. Microbiol. 2005, 71, 4117–4120. [Google Scholar] [CrossRef] [PubMed]
  37. Guan, S.Y.; Zhang, D.S.; Zhang, Z.M. Soil Enzymes and Its Methodology; Agricultural Press: Beijing, China, 1986; pp. 274–338. [Google Scholar]
  38. Liu, D.; Fang, S.; Tian, Y.; Dun, X. Variation in rhizosphere soil microbial index of tree species on seasonal flooding land: An in situ rhizobox approach. Appl. Soil Ecol. 2012, 59, 1–11. [Google Scholar] [CrossRef]
  39. Fang, S.Z.; Liu, D.; Tian, Y.; Deng, S.P.; Shang, X.L. Tree species composition influences enzyme activities and microbial biomass in the rhizosphere: A rhizobox approach. PLoS ONE 2013, 8, e61461. [Google Scholar] [CrossRef] [PubMed]
  40. Girvan, M.S.; Bullimore, J.; Pretty, J.N.; Osborn, A.M.; Ball, A.S. Soil type is the primary determinant of the composition of the total and active bacterial communities in arable soil. Appl. Environ. Microbiol. 2003, 69, 1800–1809. [Google Scholar] [CrossRef] [PubMed]
  • Sample Availability: Samples of the compounds MBC, DAZ and BOF, are available from the authors.
Molecules 21 00526 g001 550
Figure 1. Chrysanthemum Fusarium wilt incidence (a) and disease reduction percentage (DRP) (b) as affected by exposure to the various treatments for between 30 to 90 days following transplantation after three croppings. The bars represent the mean and the whiskers the standard deviation. See footnote to Table 1 for the treatment codes. Letters above the bars indicate a significant difference according to Duncan’s multiple range test at p < 0.05 level.
Figure 1. Chrysanthemum Fusarium wilt incidence (a) and disease reduction percentage (DRP) (b) as affected by exposure to the various treatments for between 30 to 90 days following transplantation after three croppings. The bars represent the mean and the whiskers the standard deviation. See footnote to Table 1 for the treatment codes. Letters above the bars indicate a significant difference according to Duncan’s multiple range test at p < 0.05 level.
Molecules 21 00526 g001
Molecules 21 00526 g002 550
Figure 2. Enzyme activity: (a) Catalase; (b) Urease; and (c) Sucrose in soils sampled from plants experiencing the range of treatments. The bars represent the mean and the whiskers the standard deviation. See footnote to Table 1 for the treatment codes. Letters above the bars indicate a significant difference according to Duncan’s multiple range test at p < 0.05 level.
Figure 2. Enzyme activity: (a) Catalase; (b) Urease; and (c) Sucrose in soils sampled from plants experiencing the range of treatments. The bars represent the mean and the whiskers the standard deviation. See footnote to Table 1 for the treatment codes. Letters above the bars indicate a significant difference according to Duncan’s multiple range test at p < 0.05 level.
Molecules 21 00526 g002
Molecules 21 00526 g003a 550Molecules 21 00526 g003b 550
Figure 3. DGGE profiling of soil microbiota: (a) the bacterial community; and (b) the fungal community. Numbered fragments were excised, re-amplified and sequenced. See footnote to Table 1 for the treatment codes.
Figure 3. DGGE profiling of soil microbiota: (a) the bacterial community; and (b) the fungal community. Numbered fragments were excised, re-amplified and sequenced. See footnote to Table 1 for the treatment codes.
Molecules 21 00526 g003aMolecules 21 00526 g003b
Table
Table 1. The effect of the various soil treatments on the growth of chrysanthemum *.
Table 1. The effect of the various soil treatments on the growth of chrysanthemum *.
TreatmentShootLeafFlower
Height (cm)Diameter (cm)Fresh wt (g/plant)Dry wt (g/plant)Width (cm)Length (cm)SPAD Value (%)Diameter (cm)Ray Floret Number (No.)
CK56.45 ± 4.01 d3.35 ± 0.32 d49.31 ± 4.75 d8.95 ± 0.89 d3.24 ± 0.31 d6.48 ± 0.55 d13.67 ± 1.73 d8.25 ± 0.46 d231.83 ± 17.34 f
MBC62.63 ± 3.32 c4.39 ± 0.41 c56.61 ± 4.57 c9.97 ± 0.73 c4.01 ± 0.24 c7.46 ± 0.46 c14.92 ± 1.66 c9.32 ± 0.52 c249.20 ± 18.56 e
DAZ63.01 ± 3.49 c4.91 ± 0.34 c57.05 ± 5.52 c10.16 ± 0.87 c4.28 ± 0.27 c7.51 ± 0.38 c14.53 ± 1.62 c9.39 ± 0.43 c259.55 ± 14.39 d
BOF69.83 ± 4.18 b5.92 ± 0.37 b68.79 ± 4.28 b14.23 ± 0.83 b5.43 ± 0.29 b8.32 ± 0.42 b17.35 ± 1.71 b10.50 ± 0.51 b261.89 ± 17.77 c
MBC + BOF69.98 ± 4.41 b5.99 ± 0.35 b69.08 ± 4.98 b14.76 ± 0.64 b5.27 ± 0.31 b8.45 ± 0.34 b17.07 ± 1.63 b10.43 ± 0.49 b269.34 ± 21.97 b
DAZ + BOF72.01 ± 3.92 a6.96 ± 0.33 a72.92 ± 4.82 a16.17 ± 0.91 a6.34 ± 0.25 a9.46 ± 0.36 a18.39 ± 1.92 a11.49 ± 0.40 a271.49 ± 19.72 a
* Data given in the form mean ± standard deviation. Treatment codes: CK: No treatment, MBC: Fungicide carbendazim treatment, DAZ: fumigation dazomet treatment, MBC + BOF: Fungicide carbendazim and BOF treatment, DAZ + BOF: fumigation dazomet and BOF treatment, BOF: Bio-organic fertilizer treatment. SPAD value means the leaf chlorophyll content. Different letters indicate significant differences among soil treatments according to Duncan’s multiple range test at p < 0.05 level.
Table
Table 2. Quantification of soil bacteria and fungi populations based on real-time PCR. Values given in the form mean ± SE (n = 3) *.
Table 2. Quantification of soil bacteria and fungi populations based on real-time PCR. Values given in the form mean ± SE (n = 3) *.
TreatmentBacteria (104 cfu·g−1 soil)Fungi (104 cfu·g−1 soil)Bacteria/Fungi Ratio
CK89.6 ± 0.59 c8.66 ± 0.39 a10.3 ± 0.9 d
MBC92.8 ± 0.48 c7.34 ± 0.37 bc13.7 ± 1.0 c
DAZ91.7 ± 0.68 c4.94 ± 0.22 d18.6 ± 1.5 b
MBC + BOF145.0 ± 0.72 b6.78 ± 0.39 b19.8 ± 1.2 b
DAZ + BOF179.0 ± 0.73 a5.86 ± 0.38 c30.5 ± 1.1 a
BOF140.0 ± 0.82 b6.47 ± 0.42 bc21.6 ± 1.2 b
* See footnote to Table 1 for the treatment codes. Different letters indicate significant differences among soil treatments according to Duncan’s multiple range test at p < 0.05 level.
Table
Table 3. Shannon diversity (H') and equitability (J) indices of the soil microbiota in response to the various soil treatments. The indices and number of fragments (n) were determined from DGGE profiles. Data given in the form mean ± SE (n = 3) *.
Table 3. Shannon diversity (H') and equitability (J) indices of the soil microbiota in response to the various soil treatments. The indices and number of fragments (n) were determined from DGGE profiles. Data given in the form mean ± SE (n = 3) *.
TreatmentsBacteriaFungi
nH′JnH′J
CK253.10 ± 0.30 a0.96 ± 0.02 a243.00 ± 0.01 a0.66 ± 0.07 d
MBC323.15 ± 0.33 a0.98 ± 0.09 a51.24 ± 0.10 f0.77 ± 0.06 c
DAZ243.12 ± 0.23 a0.98 ± 0.04 a161.65 ± 0.12 b0.81 ± 0.02 b
MBC+BOF253.14 ± 0.37 a0.97 ± 0.03 a71.81 ± 0.18 e0.89 ± 0.00 b
DAZ+BOF263.19 ± 0.11 a0.97 ± 0.02 a81.99 ± 0.20 d0.96 ± 0.01 a
BOF353.23 ± 0.22 a0.96 ± 0.11 a142.57 ± 0.11 c0.98 ± 0.04 a
* See footnote to Table 1 for the treatment codes. Different letters indicate significant differences among soil treatments according to Duncan’s multiple range test at p < 0.05 level.
Table
Table 4. Phylogeny of sequences present in the 16S rRNA amplicons (bacteria).
Table 4. Phylogeny of sequences present in the 16S rRNA amplicons (bacteria).
DGGE BandClosest Relatives Microorganisms (Phylogenic Affiliations)Similarity (%)Genebank Accession No.
1Uncultured Xanthomonadaceae bacterium96FJ53688611
2Uncultured bacterium97EU362858.1
3Uncultured Ohtaekwangia sp.97JX493344.1
4Uncultured Bacillus sp.99HQ1791481
5Alpha proteobacterium96JQ608334.2
6Uncultured Bradyrhizobium sp.95HE654679.1
7Uncultured Xanthomonadaceae bacterium97FJ88933811
8Agricultural soil bacterium98HQ132702.1
9Uncultured beta proteobacterium98AJ318162.1
10Uncultured methylovirgula sp.99KC297188.1
11Alpha proteobacterium97AB470422.1
12Rhizobium sp.95HG423545.1
13Variovorax sp.97HM484318.1
14Microbacterium sp.98AJ318162.1
15Sphingomonas sp.98JQ608334.2
16Uncultured bacterium97FJ796671.1
17Anoxybacillus flavithermus98KF279366.1
18Sphingobium yanoikuyae98GQ214010.1
19Uncultured Chloroflexi bacterium94HM164420.1
20Flavobacterium sp.100KF891387.1
21Uncultured bacterium96JQ890611.1
22Dechlorospirillum98GU167977.1
23Uncultured Flexibacter sp.96GU201555.1
24Ochrobactrum sp.97HQ659714.1
25Agricultural soil bacterium95KC193578.1
26Uncultured bacterium99EU419388.1
27Mycobacterium sp.97DQ658940.1
28Uncultured Methylocystis sp.97GU227561.1
29Mycobacterium gilvum97AJ699170.3
30Clostridium neonatale96GU227558.1
Table
Table 5. Phylogeny of sequences present in the 18S rRNA amplicons (fungi).
Table 5. Phylogeny of sequences present in the 18S rRNA amplicons (fungi).
DGGE BandClosest Relatives Microorganisms (Phylogenic Affiliations)Similarity (%)Genebank Accession No.
1Aspergillus terreus98JN639854.1
2Leptosphaeria maculans98NW003533867.1
3Fusarium sp.97EU381149.1
4Ophiobolus herpotrichus100U43453.1
5Psilocybe silvatica97DQ851583.1
6Alnicola sp.98JN939094.1
7Uncultured Clitopilus96GQ995701.1
8Mythicomyces corneipes98DQ092917.1
9Arthrobotrys oligospora98JQ809337.1
10Aspergillus ochraceus97AB002068.1
11Cucurbitaria sp.99FJ215704.1
12Phoma macrostoma97AB454217.1
13Phoma sp.97AY293772.1
14Leptosphaeria microscopica99U04235.1
15Gondwanamyces proteae97AY271804.1
16Penicillium sp.97JF950269.1
17Monodictys arctica97EU686519.1
18Graphium putredinis99AB007683.1
19Saccobolus dilutellus99FJ176814.1
20Orbilia auricolor99DQ471001.1
21Uncultured soil fungi97AJ877196

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Zhao, S.; Chen, X.; Deng, S.; Dong, X.; Song, A.; Yao, J.; Fang, W.; Chen, F. The Effects of Fungicide, Soil Fumigant, Bio-Organic Fertilizer and Their Combined Application on Chrysanthemum Fusarium Wilt Controlling, Soil Enzyme Activities and Microbial Properties. Molecules 2016, 21, 526. https://doi.org/10.3390/molecules21040526

AMA Style

Zhao S, Chen X, Deng S, Dong X, Song A, Yao J, Fang W, Chen F. The Effects of Fungicide, Soil Fumigant, Bio-Organic Fertilizer and Their Combined Application on Chrysanthemum Fusarium Wilt Controlling, Soil Enzyme Activities and Microbial Properties. Molecules. 2016; 21(4):526. https://doi.org/10.3390/molecules21040526

Chicago/Turabian Style

Zhao, Shuang, Xi Chen, Shiping Deng, Xuena Dong, Aiping Song, Jianjun Yao, Weimin Fang, and Fadi Chen. 2016. "The Effects of Fungicide, Soil Fumigant, Bio-Organic Fertilizer and Their Combined Application on Chrysanthemum Fusarium Wilt Controlling, Soil Enzyme Activities and Microbial Properties" Molecules 21, no. 4: 526. https://doi.org/10.3390/molecules21040526

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