Next Article in Journal
Synthesis and Anticancer Activities of Glycyrrhetinic Acid Derivatives
Previous Article in Journal
Biochemical Characteristics of Three Laccase Isoforms from the Basidiomycete Pleurotus nebrodensis
Article Menu
Issue 2 (February) cover image

Export Article

Open AccessArticle
Molecules 2016, 21(2), 204; doi:10.3390/molecules21020204

A Continuous Procedure Based on Column Chromatography to Purify Anthocyanins from Schisandra chinensis by a Macroporous Resin plus Gel Filtration Chromatography

1
College of Material Science and Engineering, Northeast Forestry University, Harbin 150040, China
2
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China
3
Key Laboratory of Wood Science and Technology of Zhejiang Province, Zhejiang Agriculture and Forestry University, Hangzhou, Lin’an 311300, China
*
Authors to whom correspondence should be addressed.
Received: 2 December 2015 / Accepted: 3 February 2016 / Published: 6 February 2016
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [2280 KB, uploaded 15 February 2016]   |  

Abstract

In our previous study, as natural food colorants and antioxidants, the color and content stabilities of Schisandra chinensis (S. chinensis) anthocyanins were investigated. In this work, the purification process parameters of S. chinensis anthocyanins using a macroporous resin and gel filtration chromatography were evaluated. The optimized parameters of static adsorption and desorption were as follows. The selected resin is HPD-300 (nonpolar copolymer styrene type resin), and the anthocyanins adsorption saturation capacity of HPD-300 resin was 0.475 mg/g dry resin. Adsorption time was 4 h, and 0.517 mg/mL of S. chinensis anthocyanins was adsorbed on the resin column with a flow rate of 39 mL/h (3 BV/h). After adsorption, the anthocyanins were completely desorpted with 2.5 BV of 90% (v/v) ethanol solution, and the desorption flow rate was 13 mL/h (1 BV/h). After purification by dynamic adsorption and desorption, the anthocyanins content in the effluent increased from 47.6 mg/g to 128.4 mg/g, the purity of anthocyanins increased six-fold from 5.08% to 30.43%, and the anthocyanins recovery was 96.5%. The major constituent of S. chinensis anthocyanins was isolated with Bio-Gel P2 gel filtration chromatography, and it was detected by liquid chromatography electrospray ionisation tandem mass spectrometry (LC-ESI-MS) as cyanidin-3-O-xylosylrutinoside. Moreover, the antioxidant activities of S. chinensis anthocyanins were investigated. After purification using the HPD-300 resin, the antioxidant activities of anthocyanins were increased 1.2-fold (FRAP) and 1.7-fold (ABTS). View Full-Text
Keywords: Schisandra chinensis; anthocyanins; macroporous resin; gel filtration chromatography; LC-ESI-MS; antioxidant activities Schisandra chinensis; anthocyanins; macroporous resin; gel filtration chromatography; LC-ESI-MS; antioxidant activities
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Yue, D.; Yang, L.; Liu, S.; Li, J.; Li, W.; Ma, C. A Continuous Procedure Based on Column Chromatography to Purify Anthocyanins from Schisandra chinensis by a Macroporous Resin plus Gel Filtration Chromatography. Molecules 2016, 21, 204.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top