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Molecules 2016, 21(12), 1745; doi:10.3390/molecules21121745

Characterization of Fractional Polysaccharides from Gleditsia sinensis and Gleditsia microphylla Gums

1
MOE Engineering Research Center of Forestry Biomass Materials and Bioenergy, Beijing Forestry University, Beijing 100083, China
2
Nanjing Institute for the Comprehensive Utilization of Wild Plant, Nanjing 210042, China
*
Authors to whom correspondence should be addressed.
Academic Editors: Quan-Bin Han, Sunan Wang, Shaoping Nie and Derek J. McPhee
Received: 10 October 2016 / Revised: 12 December 2016 / Accepted: 14 December 2016 / Published: 19 December 2016
(This article belongs to the Special Issue Natural Polysaccharides)
View Full-Text   |   Download PDF [906 KB, uploaded 19 December 2016]   |  

Abstract

The seeds of Gleditsia sinensis and Gleditsia microphylla, widespread in China, are an important source of galactomannans. G. sinensis gum (GSG) and G. microphylla gum (GMG) were purified and precipitated using different concentrations of ethanol and isopropanol. The GSG and GMG, precipitated in different stages, presented different characteristics, including polymer recovery, mannose/galactose ratio, chemical composition, molecular weight, and morphological appearance. The galactomannan recovery of GSG and GMG in 33.3% ethanol was 81.7% and 82.5%, respectively, while that in 28.8% isopropanol was 81.3% and 82.9%, respectively. To achieve similar precipitation efficiency, the amount of isopropanol should be lower than that of ethanol because of the lower dielectric constant of isopropanol (20 vs. 25 for ethanol). The precipitation behavior of galactomannans in polar organic solvents was dependent on the molecular structures and properties of the solvent. A higher mannose/galactose ratio and a higher molecular weight was obtained in a lower concentration of alcohols. View Full-Text
Keywords: Gleditsia sinensis; Gleditsia microphylla; ethanol; isopropanol; fractionation Gleditsia sinensis; Gleditsia microphylla; ethanol; isopropanol; fractionation
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MDPI and ACS Style

Liu, Y.; Xu, Z.; Zhang, W.; Duan, J.; Jiang, J.; Sun, D. Characterization of Fractional Polysaccharides from Gleditsia sinensis and Gleditsia microphylla Gums. Molecules 2016, 21, 1745.

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