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Molecules 2016, 21(12), 1733; doi:10.3390/molecules21121733

Validation and Application of an Ultra High-Performance Liquid Chromatography Tandem Mass Spectrometry Method for Yuanhuacine Determination in Rat Plasma after Pulmonary Administration: Pharmacokinetic Evaluation of a New Drug Delivery System

1,2,†
,
1,†
,
1,†
,
1
,
1
,
1
,
1
,
1,2
and
1,2,*
1
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
2
Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing 210023, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 14 November 2016 / Revised: 5 December 2016 / Accepted: 13 December 2016 / Published: 16 December 2016
(This article belongs to the Section Medicinal Chemistry)
View Full-Text   |   Download PDF [1398 KB, uploaded 16 December 2016]   |  

Abstract

Yuanhuacine was found to have significant inhibitory activity against A-549 human lung cancer cells. However, there would be serious adverse toxicity effects after systemic administration of yuanhuacine, such as by oral and intravenous ways. In order to achieve better curative effect and to alleviate the adverse toxicity effects, we tried to deliver yuanhuacine directly into the lungs. Ultra high-performance liquid chromatography tandem mass spectrometry (UHPLC–MS/MS) was used to detect the analyte and IS. After extraction (ether:dichloromethane = 8:1), the analyte and IS were separated on a Waters BEH-C18 column (100 mm × 2.1 mm, 1.7 μm) under a 5 min gradient elution using a mixture of acetonitrile and 0.1% formic acid aqueous solution as mobile phase at a flow rate of 0.3 mL/min. ESI positive mode was chosen for detection. The method was fully validated for its selectivity, accuracy, precision, stability, matrix effect, and extraction recovery. This new method for yuanhuacine concentration determination in rat plasma was reliable and could be applied for its preclinical and clinical monitoring purpose. View Full-Text
Keywords: yuanhuacine; pulmonary drug delivery; pharmacokinetics; ultra high-performance liquid chromatography tandem mass spectrometry yuanhuacine; pulmonary drug delivery; pharmacokinetics; ultra high-performance liquid chromatography tandem mass spectrometry
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Li, M.; Liu, X.; Cai, H.; Shen, Z.; Xu, L.; Li, W.; Wu, L.; Duan, J.; Chen, Z. Validation and Application of an Ultra High-Performance Liquid Chromatography Tandem Mass Spectrometry Method for Yuanhuacine Determination in Rat Plasma after Pulmonary Administration: Pharmacokinetic Evaluation of a New Drug Delivery System. Molecules 2016, 21, 1733.

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