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Molecules 2016, 21(12), 1707; doi:10.3390/molecules21121707

Expression of 3-Mercaptopyruvate Sulfurtransferase in the Mouse

1
Department of Pathology and Experimental Medicine, Kumamoto University Graduate School of Medical Sciences, 1-1-1 Honjo Chuo-ku, Kumamoto 860-8556, Japan
2
Isotope Research Center, Nippon Medical School; Tokyo 113-8602, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 15 November 2016 / Revised: 6 December 2016 / Accepted: 7 December 2016 / Published: 11 December 2016
(This article belongs to the Special Issue Sulfur Atom: Element for Adaptation to an Oxidative Environment 2016)
View Full-Text   |   Download PDF [3039 KB, uploaded 11 December 2016]   |  

Abstract

3-Mercaptopyruvate sulfurtransferase (MST) is one of the principal enzymes for the production of hydrogen sulfide and polysulfides in mammalians, and emerging evidence supports the physiological significance of MST. As a fundamental study of the physiology and pathobiology of MST, it is necessary to establish the tissue distribution of MST in mice. In the present study, the expression of MST in various organs of adult and fetal mice was analyzed by Western blotting and enzyme-immunohistochemistry. Moreover, the histology of MST gene–deficient mice was examined. Western blotting revealed that all organs examined had MST. The brain, liver, kidneys testes, and endocrine organs contained large amounts of MST, but the lungs, spleen, thymus, and small intestine did not. Immunohistochemically, the MST expression pattern varies in a cell-specific manner. In the brain, neural and glial cells are positively stained; in the lung, bronchiolar cells are preferentially stained; in the liver, hepatocytes around central veins are more strongly stained; renal convoluted cells are strongly stained; and pancreatic islets are strongly stained. Fetal tissues were studied, and MST expression was found to be similar before and after birth. Histological observation revealed no remarkable findings in MST gene–deficient mice. The present study revealed fundamental information regarding the MST expression of various organs in adult and fetal mice, and the morphological phenotype of MST gene–deficient mice. View Full-Text
Keywords: mercaptopyruvate methyltransferase (MST); Western blotting; immunohistochemistry; mouse mercaptopyruvate methyltransferase (MST); Western blotting; immunohistochemistry; mouse
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Tomita, M.; Nagahara, N.; Ito, T. Expression of 3-Mercaptopyruvate Sulfurtransferase in the Mouse. Molecules 2016, 21, 1707.

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