Next Article in Journal
Surface Modification of a Nanoporous Carbon Photoanode upon Irradiation
Next Article in Special Issue
Antiproliferative Effect of Indole Phytoalexins
Previous Article in Journal
The Draft Genome Sequence of Actinokineospora bangkokensis 44EHWT Reveals the Biosynthetic Pathway of the Antifungal Thailandin Compounds with Unusual Butylmalonyl-CoA Extender Units
Article Menu
Issue 11 (November) cover image

Export Article

Open AccessArticle
Molecules 2016, 21(11), 1609; doi:10.3390/molecules21111609

Simultaneous Ultra Performance Liquid Chromatography Determination and Antioxidant Activity of Linarin, Luteolin, Chlorogenic Acid and Apigenin in Different Parts of Compositae Species

1
Department of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Korea
2
Institute of Natural Medicine, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Philippe Jeandet
Received: 30 September 2016 / Revised: 11 November 2016 / Accepted: 17 November 2016 / Published: 23 November 2016
View Full-Text   |   Download PDF [969 KB, uploaded 23 November 2016]   |  

Abstract

Linarin (LA), luteolin (LE), chlorogenic acid (CA) and apigenin (AP) are four major flavonoids with various promising bioactivities found in Compositae (COP) species. A reliable, reproducible and accurate method for the simultaneous and quantitative determination of these four major flavonoids by Ultra Performance Liquid Chromatography (UPLC) analysis was developed. This method should be appropriate for the quality assurance of COP. The UPLC separation was carried out using an octadecylsilane (ODS) Hypersil (2.1 mm × 250 mm, 1.9 μm) and a mobile phase composed of acetonitrile and 0.1% formic acid in water at a flow rate 0.44 mL/min and ultraviolet (UV) detection 254 nm. Gradient elution was employed. The method was precise, with relative standard deviation below 3.0% and showed excellent linearity (R2 > 0.999). The recoveries for the four flavonoids in COP were between 95.49%–106.23%. The average contents of LA, LE, CA and AP in different parts (flower, leave and stem) of COP were between 0.64–1.47 g/100 g, 0.66–0.89 g/100 g, 0.32–0.52 g/100 g and 0.16–0.18 g/100 g, respectively. The method was accurate and reproducible and it can provide a quantitative basis for quality control of COP. View Full-Text
Keywords: UPLC; linarin; luteolin; chlorogenic; apigenin; Compositae UPLC; linarin; luteolin; chlorogenic; apigenin; Compositae
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Hwang, S.H.; Paek, J.H.; Lim, S.S. Simultaneous Ultra Performance Liquid Chromatography Determination and Antioxidant Activity of Linarin, Luteolin, Chlorogenic Acid and Apigenin in Different Parts of Compositae Species. Molecules 2016, 21, 1609.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top