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Molecules 2016, 21(11), 1546; doi:10.3390/molecules21111546

Unfolding/Refolding Study on Collagen from Sea Cucumber Based on 2D Fourier Transform Infrared Spectroscopy

1
School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China
2
National Engineering Research Center of Seafood, Dalian 116034, China
3
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 16 September 2016 / Revised: 2 November 2016 / Accepted: 11 November 2016 / Published: 16 November 2016
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Abstract

We aimed to explore the differences of thermal behaviors between insoluble collagen fibrils (ICFs) and pepsin-solubilized collagens (PSCs) from sea cucumber Stichopus japonicus. The unfolding/refolding sequences of secondary structures of ICFs and PSCs during the heating and cooling cycle (5 → 70 → 5 °C) were identified by Fourier transform infrared spectrometry combined with curve-fitting and 2D correlation techniques. ICFs showed a higher proportion of α-helical structures and higher thermostability than PSCs, and thus had more-stable triple helical structures. The sequences of changes affecting the secondary structures during heating were essentially the same between ICFs and PSCs. In all cases, α-helix structure was the most important conformation and it disappeared to form a β-sheet structure. In the cooling cycle, ICFs showed a partially refolding ability, and the proportion of β-sheet structure rose before the increasing proportion of α-helix structure. PSCs did not obviously refold during the cooling stage. View Full-Text
Keywords: insoluble collagen fibrils; pepsin-solubilized collagens; 2D Fourier transform infrared spectrometry; thermostability; secondary structures insoluble collagen fibrils; pepsin-solubilized collagens; 2D Fourier transform infrared spectrometry; thermostability; secondary structures
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MDPI and ACS Style

Qin, L.; Bi, J.-R.; Li, D.-M.; Dong, M.; Zhao, Z.-Y.; Dong, X.-P.; Zhou, D.-Y.; Zhu, B.-W. Unfolding/Refolding Study on Collagen from Sea Cucumber Based on 2D Fourier Transform Infrared Spectroscopy. Molecules 2016, 21, 1546.

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