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Molecules 2016, 21(11), 1495; doi:10.3390/molecules21111495

Feasibility of Fraction Collection in HPLC Systems with Evaporative Light Scattering Detector: Analysis of Pectinatella magnifica

1
Department of Chemical Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackého tr. 1946/1, Brno 61242, Czech Republic
2
Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackého tr. 1946/1, Brno 61242, Czech Republic
*
Author to whom correspondence should be addressed.
Academic Editors: Josef Jampílek and Atanas G. Atanasov
Received: 5 September 2016 / Revised: 28 October 2016 / Accepted: 30 October 2016 / Published: 8 November 2016
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Abstract

The use of a liquid chromatography (LC) splitter inserted between an HPLC column and an evaporative light scattering detector (ELSD) is described. This paper aims to show the feasibility of using the splitter in an HPLC-ELSD system to fractionate a model mixture of analytes, namely salicin (2-(hydroxymethyl)-phenyl-β-d-glucopyranoside) and glucose. The retention factors and efficiency of the separation were studied under various temperatures and water contents in the mobile phase in order to clarify the mechanism of polyols separation on a diol column under the conditions of hydrophilic liquid chromatography (HILIC). Finally, the system was applied to a biological sample (a lyophilized colony gel of Pectinatella magnifica), where the presence of fructose and glucose was confirmed. View Full-Text
Keywords: fraction collection; evaporative light scattering detector; hydrophilic interaction liquid chromatography; mixed-mode chromatography; Pectinatella magnifica fraction collection; evaporative light scattering detector; hydrophilic interaction liquid chromatography; mixed-mode chromatography; Pectinatella magnifica
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Pazourek, J.; Šmejkal, K. Feasibility of Fraction Collection in HPLC Systems with Evaporative Light Scattering Detector: Analysis of Pectinatella magnifica. Molecules 2016, 21, 1495.

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