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Molecules 2016, 21(10), 1379; doi:10.3390/molecules21101379

Transcriptome Profile Reveals that Pu-Erh Tea Represses the Expression of Vitellogenin Family to Reduce Fat Accumulation in Caenorhabditis elegans

1
Pharmaceutical College, Heilongjiang University of Chinese Medicine, #24Heping Road, Harbin 150040, China
2
State Key Laboratory of Genetic Resources and Evolutionary & Functional Genomics, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China
3
Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China
4
Pharmaceutical College, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China
5
Department of Life Science and Biotechnology, Key Laboratory of Special Biological Resource Development and Utilization of University in Yunnan Province, Kunming University, Kunming 650214, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 30 August 2016 / Revised: 4 October 2016 / Accepted: 12 October 2016 / Published: 17 October 2016
(This article belongs to the Special Issue Natural Products in Anti-Obesity Therapy)
View Full-Text   |   Download PDF [3148 KB, uploaded 17 October 2016]   |  

Abstract

Due to misbalanced energy surplus and expenditure, obesity has become a common chronic disorder that is highly associated with many metabolic diseases. Pu-erh tea, a traditional Chinese beverage, has been believed to have numerous health benefits, such as anti-obesity. However, the underlying mechanisms of its anti-obesity effect are yet to be understood. Here, we take the advantages of transcriptional profile by RNA sequencing (RNA-Seq) to view the global gene expression of Pu-erh tea. The model organism Caenorhabditis elegans was treated with different concentrations of Pu-erh tea water extract (PTE, 0 g/mL, 0.025 g/mL, and 0.05 g/mL). Compared with the control, PTE indeed decreases lipid droplets size and fat accumulation. The high-throughput RNA-Sequence technique detected 18073 and 18105 genes expressed in 0.025 g/mL and 0.05 g/mL PTE treated groups, respectively. Interestingly, the expression of the vitellogenin family (vit-1, vit-2, vit-3, vit-4 and vit-5) was significantly decreased by PTE, which was validated by qPCR analysis. Furthermore, vit-1(ok2616), vit-3(ok2348) and vit-5(ok3239) mutants are insensitive to PTE triggered fat reduction. In conclusion, our transcriptional profile by RNA-Sequence suggests that Pu-erh tea lowers the fat accumulation primarily through repression of the expression of vit(vitellogenin) family, in addition to our previously reported (sterol regulatory element binding protein) SREBP-SCD (stearoyl-CoA desaturase) axis. View Full-Text
Keywords: Pu-erh tea water extract (PTE); fat accumulation; RNA-Seq; vitellogenin (vit) family Pu-erh tea water extract (PTE); fat accumulation; RNA-Seq; vitellogenin (vit) family
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Xiao, R.-Y.; Hao, J.; Ding, Y.-H.; Che, Y.-Y.; Zou, X.-J.; Liang, B. Transcriptome Profile Reveals that Pu-Erh Tea Represses the Expression of Vitellogenin Family to Reduce Fat Accumulation in Caenorhabditis elegans. Molecules 2016, 21, 1379.

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