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Molecules 2016, 21(10), 1377; doi:10.3390/molecules21101377

A Novel Tetrahydrocannabinol Electrochemical Nano Immunosensor Based on Horseradish Peroxidase and Double-Layer Gold Nanoparticles

1,2,3,4
,
1,2
and
1,2,3,4,*
1
College of Biotechnology, Tianjin University of Science &Technology, Tianjin 300457, China
2
Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin 300457, China
3
College of Biotechnology & Food Science, Tianjin University of Commerce, Tianjin 300314, China
4
Tianjin Key Laboratory of Food Biotechnology, Tianjin 300314, China
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 8 September 2016 / Revised: 9 October 2016 / Accepted: 12 October 2016 / Published: 17 October 2016
View Full-Text   |   Download PDF [5083 KB, uploaded 17 October 2016]   |  

Abstract

In the current study, a novel double-layer gold nanoparticles-electrochemical immunosensor electrode immobilized with tetrahydrocannabinol (THC) antibody derived from Balb/c mice was developed. To increase the fixed quantity of antibodies and electrochemical signals, an electrochemical biosensing signal amplification system was utilized with gold nanoparticles-thionine-chitosan absorbing horseradish peroxidase (HRP). In addition, a transmission electron microscope (TEM) was used to characterize the nanogold solution. To evaluate the quality of the immunosensor, the amperometric I-t curve method was applied to determine the THC in PBS. The results showed that the response current had a good linear correlation with the THC concentration range from 0.01~103 ng/mL with a correlation coefficient of 0.9986. The lowest detection limit for THC was 3.3 pg/mL (S/N = 3). Moreover, it was validated with high sensitivity and reproducibility. Apparently, the immunosensor may be a very useful tool for monitoring the THC. View Full-Text
Keywords: tetrahydrocannabinol (THC); electrochemical; immunosensor; amperometric I-t curve method; gold nanoparticles (GNPs) tetrahydrocannabinol (THC); electrochemical; immunosensor; amperometric I-t curve method; gold nanoparticles (GNPs)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Lu, D.; Lu, F.; Pang, G. A Novel Tetrahydrocannabinol Electrochemical Nano Immunosensor Based on Horseradish Peroxidase and Double-Layer Gold Nanoparticles. Molecules 2016, 21, 1377.

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