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Molecules 2016, 21(10), 1335; doi:10.3390/molecules21101335

Optimization Extraction, Preliminary Characterization and Antioxidant Activities of Polysaccharides from Semen Juglandis

1
College of Materials Science and Technology, Beijing Forestry University, Beijing 100083, China
2
Key Laboratory of Biological and Chemical Utilization of Zhejiang Forest Resources, Department of Forest Foods, Zhejiang Forestry Academy, Hangzhou 310023, China
*
Author to whom correspondence should be addressed.
Academic Editor: Jandirk Sendker
Received: 18 August 2016 / Revised: 23 September 2016 / Accepted: 29 September 2016 / Published: 9 October 2016
(This article belongs to the Section Natural Products)
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Abstract

The optimization extraction process, preliminary characterization and antioxidant activities of polysaccharides from Semen Juglandis (SJP) were studied in this paper. Based on the Box-Behnken experimental design and response surface methodology, the optimal extraction conditions for the SJP extraction were obtained as follows: temperature 88 °C, extraction time 125 min and ratio of liquid to solid 31 mL/g. Under these conditions, experimental extraction yield of SJP was (5.73 ± 0.014)% (n = 5), similar to the predicted value of 5.78%. Furtherly, the purified SJP obtained from SJP extract by DEAE-52 and Sephacryl S-100 chromatography was analyzed to be rhamnose, galacturonic acid, galactose, arabinose and fucose in the molar ratio of 1:6.34:1.38:3.21:1.56. And the weight-average molecular weight and radius of gyration of the purified SJP in 0.1 M NaCl were determined to be 2.76 × 104 g/mol and 122 nm by SEC-MALLS, respectively. More importantly, it exhibited appreciable antioxidant activities compared to the standard Vc, such as DPPH radical scavenging activity (IC50 0.21 mg/mL), strong reducing power, ABTS radical scavenging activity (IC50 0.29 mg/mL), and hydroxyl radical scavenging activity (IC50 0.38 mg/mL). These results indicate that SJP may be useful for developing functional health products or natural antioxidant. View Full-Text
Keywords: Semen Juglandis; polysaccharide; response surface methodology (RSM); physicochemical property; antioxidant activities Semen Juglandis; polysaccharide; response surface methodology (RSM); physicochemical property; antioxidant activities
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Ren, X.; He, L.; Wang, Y.; Cheng, J. Optimization Extraction, Preliminary Characterization and Antioxidant Activities of Polysaccharides from Semen Juglandis. Molecules 2016, 21, 1335.

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