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Molecules 2016, 21(10), 1329; doi:10.3390/molecules21101329

Quantitative Determination of 3-O-Acetyl-11-Keto-βBoswellic Acid (AKBA) and Other Boswellic Acids in Boswellia sacra Flueck (syn. B. carteri Birdw) and Boswellia serrata Roxb

1
Biosfered S.r.l., Innovation Centre, Academic Spin-Off of the University of Turin, Via Quarello 15/A, Turin 10135, Italy
2
Department of Life Sciences and Systems Biology, Innovation Centre, University of Turin, Via Quarello 15/A, Turin 10135, Italy
*
Author to whom correspondence should be addressed.
Received: 29 July 2016 / Accepted: 1 October 2016 / Published: 6 October 2016
(This article belongs to the Special Issue Triterpenes and Triterpenoids 2016)
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Abstract

Boswellia serrata and Boswellia sacra (syn. B. carteri) are important medicinal plants widely used for their content of bioactive lipophilic triterpenes. The qualitative and quantitative determination of boswellic acids (BAs) is important for their use in dietary supplements aimed to provide a support for osteoarthritic and inflammatory diseases. We used High Performance Liquid Chromatography (HPLC)-Diode Array Detector (DAD) coupled to ElectroSpray Ionization and tandem Mass Spectrometry (ESI-MS/MS) for the qualitative and quantitative determination of BAs extracted from the gum resins of B. sacra and B. serrata. Limit of detection (LOD), limit of quantification (LOQ), and Matrix Effect were assessed in order to validate quantitative data. Here we show that the BAs quantitative determination was 491.20 g·kg−1 d. wt (49%) in B. sacra and 295.25 g·kg−1 d. wt (30%) in B. serrata. Lower percentages of BAs content were obtained when BAs were expressed on the gum resin weight (29% and 16% for B. sacra and B. serrata, respectively). The content of Acetyl-11-Keto-β-Boswellic Acid (AKBA) was higher in B. sacra (70.81 g·kg−1 d. wt; 7%) than in B. serrata (7.35 g·kg−1 d. wt; 0.7%). Our results show that any claim of BAs content in either B. sacra or B. serrata gum resins equal to or higher than 70% or AKBA contents of 30% are simply unrealistic or based on a wrong quantitative determination. View Full-Text
Keywords: Acetyl-11-Keto-β-Boswellic Acid; 11-Keto-beta-Boswellic Acid; boswellic acids; standardization; Boswellia serrata; Boswellia sacra; HPLC-DAD-ESI-MS/MS Acetyl-11-Keto-β-Boswellic Acid; 11-Keto-beta-Boswellic Acid; boswellic acids; standardization; Boswellia serrata; Boswellia sacra; HPLC-DAD-ESI-MS/MS
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Mannino, G.; Occhipinti, A.; Maffei, M.E. Quantitative Determination of 3-O-Acetyl-11-Keto-βBoswellic Acid (AKBA) and Other Boswellic Acids in Boswellia sacra Flueck (syn. B. carteri Birdw) and Boswellia serrata Roxb. Molecules 2016, 21, 1329.

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