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Molecules 2016, 21(1), 97; doi:10.3390/molecules21010097

Analysis on the Physicochemical Properties of Ginkgo biloba Leaves after Enzymolysis Based Ultrasound Extraction and Soxhlet Extraction

1,2,3,4,5,* and 1,2,3,4
Institute of Chemical Industry of Forest Products, CAF, Nanjing 210042, Jiangsu, China
National Engineering Laboratory for Biomass Chemical Utilization, Nanjing 210042, Jiangsu, China
Key and Open Laboratory on Forest Chemical Engineering, SFA, Nanjing 210042, Jiangsu, China
Key Laboratory of Biomass Energy and Material, Nanjing 210042, Jiangsu, China
Institute of New Technology of Forestry, CAF, Beijing 100091, China
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 13 October 2015 / Revised: 5 January 2016 / Accepted: 11 January 2016 / Published: 15 January 2016
(This article belongs to the Section Molecular Diversity)
View Full-Text   |   Download PDF [3001 KB, uploaded 15 January 2016]   |  


In this study, high performance liquid chromatography (HPLC), ultraviolet (UV), thermagravimetric analyzer (TGA), pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS), and scanning electron microscope (SEM) were used as measurement techniques, contents of chemical composition, pyrolytic products, thermal stability, morphological characterization of Ginkgo biloba leaves (GBL) acted as the index, and physicochemical properties of GBL after enzymolysis based ultrasound extraction (EBUE) and Soxhlet extraction were studied. The detection results of chemical composition revealed that contents of general flavone, soluble protein, soluble total sugar and protein in the GBL declined significantly after EBUE, and contents of polyprenols and crude fat obviously reduced as well after Soxhlet extraction. Py-GC-MS results indicated that total GC contents of micromolecules with carbon less than 12 from 54.0% before EBUE decline to 8.34% after EBUE. Total GC contents of long-chain fatty acids with carbon less than 20 from 43.0% before EBUE reduced to 27.0% after Soxhlet extraction. Thermal stability results showed that GBL after Soxhlet extraction was easier to decompose than GBL before EBUE. SEM results illustrated that surface structure of GBL was damaged severely after EBUE, compared with GBL before EBUE, while organic solvent extraction had little influence on the morphological characterization of GBL after Soxhlet extraction compared with GBL after EBUE. View Full-Text
Keywords: physicochemical properties; Ginkgo biloba leaves; enzymolysis; ultrasound; Soxhlet extraction physicochemical properties; Ginkgo biloba leaves; enzymolysis; ultrasound; Soxhlet extraction

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Zhang, C.-W.; Wang, C.-Z.; Tao, R. Analysis on the Physicochemical Properties of Ginkgo biloba Leaves after Enzymolysis Based Ultrasound Extraction and Soxhlet Extraction. Molecules 2016, 21, 97.

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