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Molecules 2015, 20(8), 14849-14859; doi:10.3390/molecules200814849

Structural Stabilities and Transformation Mechanism of Rhynchophylline and Isorhynchophylline by Ultra Performance Liquid Chromatography/Time-of-Flight Mass Spectrometry (UPLC/Q-TOF-MS)

1
Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
2
Luzhou People's Hospital, Luzhou 646100, China
3
Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 16 June 2015 / Accepted: 10 August 2015 / Published: 14 August 2015
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [804 KB, uploaded 14 August 2015]   |  

Abstract

To reveal the structural stabilities and transformation mechanism of rhynchophylline (RIN) and isorhynchophylline (IRN), HPLC and UPLC-Q-TOF-MS method were developed for the qualitative and quantitative analysis of the conversion rate. The method was validated for linearity, inter- and intra-day precisions, repeatability and stability. All the quantitative determination method validation results were satisfactory. Under the optimized chromatographic conditions, the effect of various heat temperatures, retention time, and solvent polarities on conversion rate and equilibrium were systematically investigated for the first time. Besides, a model relating the retention yield value and time-temperature was built to predict the t0.5 and Ea of the conversion rate by the Arrhenius equation. The experimental results proved to be in good accordance with the predicted values. Furthermore, UPLC-Q-TOF-MS analysis was performed to verify the transformation mechanism and provide valuable information for stability analysis of the conversion products. View Full-Text
Keywords: rhynchophylline; isorhynchophylline; conversion rate; stability; UPLC-Q-TOF-MS rhynchophylline; isorhynchophylline; conversion rate; stability; UPLC-Q-TOF-MS
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Wu, Z.-F.; Wang, Y.-Q.; Wan, N.; Ke, G.; Yue, P.-F.; Chen, H.; Zhan, J.-J.; Yang, M. Structural Stabilities and Transformation Mechanism of Rhynchophylline and Isorhynchophylline by Ultra Performance Liquid Chromatography/Time-of-Flight Mass Spectrometry (UPLC/Q-TOF-MS). Molecules 2015, 20, 14849-14859.

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