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Molecules 2015, 20(7), 12450-12458; doi:10.3390/molecules200712450

Potential Mechanism of Action of 3′-Demethoxy-6-O-demethyl-isoguaiacin on Methicillin Resistant Staphylococcus aureus

1
Facultad de Ciencias Químicas, Universidad Autónoma de Nuevo León, Av. Universidad S/N Ciudad Universitaria, San Nicolás de los Garza, Nuevo León C.P. 66451, Mexico
2
Facultad de Ciencia Químicas, Universidad Juárez del Estado de Durango, Av. Artículo 123 S/N, Núcleo Universitario, Col. Filadelfia, Gómez Palacio, Durango C.P. 35015, Mexico
3
Servicio de Gastroenterología y Departamento de Patología Clínica, Hospital Universitario, Dr. José Eleuterio González. Madero y Aguirre Pequeño, Universidad Autónoma de Nuevo León, Mitras Centro, Monterrey, Nuevo León C.P. 64460, Mexico
*
Author to whom correspondence should be addressed.
Academic Editor: Peter J. Rutledge
Received: 18 March 2015 / Revised: 1 July 2015 / Accepted: 2 July 2015 / Published: 8 July 2015
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Abstract

Bacterial infections represent one of the main threats to global public health. One of the major causative agents associated with high morbidity and mortality infections in hospitals worldwide is methicillin-resistant Staphylococcus aureus. Therefore, there is a need to develop new antibacterial agents to treat these infections, and natural products are a rich source of them. In previous studies, we reported that lignan 3′-demethoxy-6-O-demethylisoguaiacin, isolated and characterized from Larrea tridentate, showed the best activity towards methicillin-resistant S. aureus. Thus, the aim of this study was to determine the potential molecular mechanism of the antibacterial activity of 3′-demethoxy-6-O-demethylisoguaiacin against methicillin-resistant S. aureus using microarray technology. Results of microarray genome expression were validated by real-time polymerase chain reaction (RT-PCR). The genetic profile expression results showed that lignan 3′-demethoxy-6-O-demethylisoguaiacin had activity on cell membrane affecting proteins of the ATP-binding cassette (ABC) transport system causing bacteria death. This molecular mechanism is not present in any antibacterial commercial drug and could be a new target for the development of novel antibacterial agents. View Full-Text
Keywords: Larrea tridentata; 3′-demethoxy-6-O-demethylisoguaiacin; methicillin-resistant Staphylococcus aureus; microarrays; real time PCR; mode of action Larrea tridentata; 3′-demethoxy-6-O-demethylisoguaiacin; methicillin-resistant Staphylococcus aureus; microarrays; real time PCR; mode of action
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Favela-Hernández, J.M.J.; Clemente-Soto, A.F.; Balderas-Rentería, I.; Garza-González, E.; Camacho-Corona, M.R. Potential Mechanism of Action of 3′-Demethoxy-6-O-demethyl-isoguaiacin on Methicillin Resistant Staphylococcus aureus. Molecules 2015, 20, 12450-12458.

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