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Molecules 2015, 20(4), 7048-7058; doi:10.3390/molecules20047048

Preparative Isolation and Purification of Lignans from Justicia procumbens Using High-Speed Counter-Current Chromatography in Stepwise Elution Mode

1
Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China
2
Laboratory of Marine Natural Products, School of Marine Sciences, Ningbo University, Ningbo 315211, China
3
College of Pharmacy, Jinan University, Guangzhou 510632, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 8 March 2015 / Revised: 3 April 2015 / Accepted: 8 April 2015 / Published: 20 April 2015
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [743 KB, uploaded 20 April 2015]   |  

Abstract

Lignans, which are recognized as main constituents in Justicia procumbens, have attracted considerable attention due to their pharmacological activities, including antitumor, anti-hepatitic, cytotoxic, anti-microbial, and anti-virus properties. Preparative high-speed counter-current chromatography (HSCCC) was successfully applied to the isolation and purification of four lignans (justicidin B (1), justicidin A (2), 6'-hydroxyjusticidin C (3) and lignan J1 (4)) from J. procumbens using stepwise elution with a pair of two-phase solvent systems composed of n-hexane–ethyl acetate–methanol–water at (1.3:1:1.3:1, v/v) and (2.5:1:2.5:1, v/v). The preparative HSCCC separation was performed on 300 mg of crude sample yielding compounds 1 (19.7 mg), 2 (9.86 mg), 3 (11.26 mg), and 4 (2.54 mg) in a one-step separation, with purities over 95% as determined by HPLC. The structures of these compounds were identified by MS, 1H-NMR and 13C-NMR. This is the first report on the application of HSCCC to the efficient separation of lignans from J. procumbens. View Full-Text
Keywords: Justicia procumbens; lignans; high-speed counter-current chromatography; preparative separation; stepwise elution mode Justicia procumbens; lignans; high-speed counter-current chromatography; preparative separation; stepwise elution mode
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Zhou, P.; Luo, Q.; Ding, L.; Fang, F.; Yuan, Y.; Chen, J.; Zhang, J.; Jin, H.; He, S. Preparative Isolation and Purification of Lignans from Justicia procumbens Using High-Speed Counter-Current Chromatography in Stepwise Elution Mode. Molecules 2015, 20, 7048-7058.

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