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Molecules 2015, 20(3), 5024-5037; doi:10.3390/molecules20035024

Secondary Metabolite Localization by Autofluorescence in Living Plant Cells

1
Institut des Sciences de l'Evolution Montpellier ISE-M, Université Montpellier, CNRS, IRD, EPHE, CC 065, Place Eugène Bataillon, 34095 Montpellier, France
2
Histocytology and Plant Cell Imaging platform PHIV, UMR AGAP (CIRAD, INRA, SupAgro)-UMR B&PMP (INRA, CNRS, SupAgro, Montpellier University), 34095 Montpellier, France
*
Author to whom correspondence should be addressed.
Academic Editor: Kevin D. Belfield
Received: 3 June 2014 / Revised: 9 February 2015 / Accepted: 25 February 2015 / Published: 19 March 2015
(This article belongs to the Special Issue Fluorescent Probes)
View Full-Text   |   Download PDF [3125 KB, uploaded 19 March 2015]   |  

Abstract

Autofluorescent molecules are abundant in plant cells and spectral images offer means for analyzing their spectra, yielding information on their accumulation and function. Based on their fluorescence characteristics, an imaging approach using multiphoton microscopy was designed to assess localization of the endogenous fluorophores in living plant cells. This method, which requires no previous treatment, provides an effective experimental tool for discriminating between multiple naturally-occurring fluorophores in living-tissues. Combined with advanced Linear Unmixing, the spectral analysis extends the possibilities and enables the simultaneous detection of fluorescent molecules reliably separating overlapping emission spectra. However, as with any technology, the possibility for artifactual results does exist. This methodological article presents an overview of the applications of tissular and intra-cellular localization of these intrinsic fluorophores in leaves and fruits (here for coffee and vanilla). This method will provide new opportunities for studying cellular environments and the behavior of endogenous fluorophores in the intracellular environment. View Full-Text
Keywords: autofluorescence; secondary metabolites; phenolic compounds; hydroxycinnamic acids; plant tissue; spectral imaging; Linear Unmixing autofluorescence; secondary metabolites; phenolic compounds; hydroxycinnamic acids; plant tissue; spectral imaging; Linear Unmixing
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Talamond, P.; Verdeil, J.-L.; Conéjéro, G. Secondary Metabolite Localization by Autofluorescence in Living Plant Cells. Molecules 2015, 20, 5024-5037.

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