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Molecules 2015, 20(3), 4813-4832; doi:10.3390/molecules20034813

In Vitro Neuroprotective Activities of Compounds from Angelica shikokiana Makino

1
Division of Systematic Forest and Forest Products Sciences, Department of agroenvironmental sciences, Faculty of Agriculture, Graduate School of Kyushu University, Fukouka 812-8581, Japan
2
Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt
3
Department of Bioscience & Biotechnology, Graduate School of Bioresource and Bioenvironmental sciences, Kyushu University, Fukouka 812-8581, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Peter Koulen
Received: 5 February 2015 / Revised: 3 March 2015 / Accepted: 9 March 2015 / Published: 16 March 2015
View Full-Text   |   Download PDF [2012 KB, uploaded 16 March 2015]   |  

Abstract

Angelica shikokiana is widely marketed in Japan as a dietary food supplement. With a focus on neurodegenerative conditions such as Alzheimer’s disease, the aerial part was extracted and through bio-guided fractionation, fifteen compounds [α-glutinol, β-amyrin, kaempferol, luteolin, quercetin, kaempferol-3-O-glucoside, kaempferol-3-O-rutinoside, methyl chlorogenate, chlorogenic acid, hyuganin E, 5-(hydroxymethyl)-2-furaldehyde, β-sitosterol-3-O-glucoside, adenosine (isolated for the first time from A. shikokiana), isoepoxypteryxin and isopteryxin] were isolated. Isolated compounds were evaluated for in vitro neuroprotection using acetylcholine esterase inhibitory, protection against hydrogen peroxide and amyloid β peptide (Aβ25-35)-induced neurotoxicity in neuro-2A cells, scavenging of hydroxyl radicals and intracellular reactive oxygen species and thioflavin T assays. Quercetin showed the strongest AChE inhibition (IC50 value = 35.5 µM) through binding to His-440 and Tyr-70 residues at the catalytic and anionic sites of acetylcholine esterase, respectively. Chlorogenic acid, its methyl ester, quercetin and luteolin could significantly protect neuro-2A cells against H2O2-induced neurotoxicity and scavenge hydroxyl radical and intracellular reactive oxygen species. Kaempferol-3-O-rutinoiside, hyuganin E and isoepoxypteryxin significantly decreased Aβ25-35-induced neurotoxicity and Th-T fluorescence. To the best of our knowledge, this is the first report about neuroprotection of hyuganin E and isoepoxypteryxin against Aβ25-35-induced neurotoxicity. View Full-Text
Keywords: Angelica shikokiana; neuroprotection; amyloid β peptide; hydrogen peroxide; acetylcholine esterase Angelica shikokiana; neuroprotection; amyloid β peptide; hydrogen peroxide; acetylcholine esterase
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Mira, A.; Yamashita, S.; Katakura, Y.; Shimizu, K. In Vitro Neuroprotective Activities of Compounds from Angelica shikokiana Makino. Molecules 2015, 20, 4813-4832.

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