Next Article in Journal
Natural Products for the Treatment of Trachoma and Chlamydia trachomatis
Previous Article in Journal
Tethering in RNA: An RNA-Binding Fragment Discovery Tool
Article Menu

Export Article

Open AccessArticle
Molecules 2015, 20(3), 4162-4179; doi:10.3390/molecules20034162

Chemical Constituents and Structural Characterization of Polysaccharides from Four Typical Bamboo Species Leaves

1
Institute of Chemical Industry of Forest Products, CAF, Nanjing 210042, China
2
National Engineering Laboratory for Biomass Chemical Utilization, Nanjing 210042, China
3
Key Laboratory of Biomass Energy and Material, Nanjing 210042, China
4
Institute of New Technology of Forestry, CAF, Beijing 100091, China
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 1 December 2014 / Revised: 14 January 2015 / Accepted: 12 February 2015 / Published: 5 March 2015
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [815 KB, uploaded 5 March 2015]   |  

Abstract

In order to find bamboo leaves with high contents of bioactive polysaccharides, 32 samples were chosen to analyze their polysaccharide content by GC and sulfuric acid-anthrone colorimetric assays. Purified polysaccharides (BLPS) were separated from the four varieties P. nigra (Lodd.) Munro (PN), P. vivax McClure (PV), Chimonobambusa quadrangularis (Fenzi) Makino (CQ), and P. bambussoides cv. Tanakae (PB) by ultrasound extraction, solution precipitation, ion exchange resin, DEAE-52 and Sephadex G-100 chromatography. BLPS structural characterization was accomplished by HPLC-GPC, Fourier transform infra-red spectroscopy (FTIR) and NaIO4-HIO4 oxidation reactions. The results showed that the total polysaccharides of the bamboo leaves in samples 1–32 ranged between 1.4% and 5.4%, Samples No. 29–No. 32 (PN, PV, CQ, and PB) contained 2–3 fold more polysaccharides than No. 1~No. 28 among the 32 different species, particularly the content of galactose was in a range of 21.5%–34.1% for these four typical bamboo species leaves, which was also more than 2–3 fold higher than in No. 1–No. 28. Sugar analysis indicated that PN-PBLPS-1, PV-PBLPS-1, CQ-PBLPS-1 and PB-PBLPS-1 from the four varieties were homogeneous polysaccharides with molecular weights of 2.04 × 104, 1.15 × 104, 8.75 × 104 and 1.48 × 104 Da, respectively. PB-PBLPS-1 was a mixture of α-galactopyranose and β-d-glucopyranose linkages with α-(1→6) or β-(1→6)glycosidic bonds, while PN-PBLPS-1, PV-PBLPS-1, and CQ-PBLPS-1 had α galactopyranose linkages with α-(1→6) glycosidic bonds. View Full-Text
Keywords: bamboo leaves; polysaccharides; HPLC-GPC; FTIR; NaIO4-HIO4 bamboo leaves; polysaccharides; HPLC-GPC; FTIR; NaIO4-HIO4
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Wang, C.-Z.; Zhang, H.-Y.; Li, W.-J.; Ye, J.-Z. Chemical Constituents and Structural Characterization of Polysaccharides from Four Typical Bamboo Species Leaves. Molecules 2015, 20, 4162-4179.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top