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Molecules 2015, 20(3), 3697-3715; doi:10.3390/molecules20033697

Bioassay-Guided Fractionation of Melastoma malabathricum Linn. Leaf Solid Phase Extraction Fraction and Its Anticoagulant Activity

1
Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
2
Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
3
Institute of Biosciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
4
Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 19 November 2014 / Revised: 17 December 2014 / Accepted: 9 January 2015 / Published: 24 February 2015
(This article belongs to the Section Natural Products)
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Abstract

The aims of this study were to examine the bioactive component(s) responsible for the anticoagulant activity of M. malabathricum Linn. leaf hot water crude extract via bioassay-guided fractionation and to evaluate the effect of bioactive component(s) on the intrinsic blood coagulation pathway. The active anticoagulant fraction of F3 was subjected to a series of chromatographic separation and spectroscopic analyses. Furthermore, the effect of the bioactive component(s) on the intrinsic blood coagulation pathway was studied through immediate and time incubation mixing studies. Through Activated Partial Thromboplastin Time (APTT) assay-guided fractionation, Subfraction B was considered the most potent anticoagulant fraction. Characterisation of Subfraction B indicated that anticoagulant activity could partly be due to the presence of cinnamic acid and a cinnamic acid derivative. APTT assays for both the immediate and time incubation mixing were corrected back into normal clotting time range (35.4–56.3 s). In conclusion, cinnamic acid and cinnamic acid derivative from Subfraction B were the first such compounds to be discovered from M. malabathricum Linn. leaf hot water crude extract that possess anticoagulant activity. This active anticoagulant Subfraction B prolonged blood clotting time by causing factor(s) deficiency in the intrinsic blood coagulation pathway. View Full-Text
Keywords: bioassay-guided fractionation; Melastoma malabathricum; anticoagulant; cinnamic acid; cinnamic acid derivative; mixing study bioassay-guided fractionation; Melastoma malabathricum; anticoagulant; cinnamic acid; cinnamic acid derivative; mixing study
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Khoo, L.T.; Abdullah, J.O.; Abas, F.; Tohit, E.R.M.; Hamid, M. Bioassay-Guided Fractionation of Melastoma malabathricum Linn. Leaf Solid Phase Extraction Fraction and Its Anticoagulant Activity. Molecules 2015, 20, 3697-3715.

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